Fei Lingyu, Hou Ziying, Wang Yuan, Sun Jikang, An Tingting, Li Qiuyun
College of Life and Environmental Sciences, Central South University of Forestry and Technology, Changsha, Hunan China.
Physiol Mol Biol Plants. 2025 Feb;31(2):299-310. doi: 10.1007/s12298-025-01552-y. Epub 2025 Jan 24.
The slow growth rate of Hemsl. (Zanthoxylum) is the important factor causing the scarcity of its available wild resource. It has been reported that the plant endophytes can promote the plant growth and the synthesis of secondary metabolitesby by enhancing the efficiency of nutrient absorption by plants and regulating plant hormones. It is important to explore the promoting effects of endophytes on the growth of Zanthoxylum. The application of high-throughput sequencing technology in this study revealed the presence of three phyla, five classes, seven orders, and eleven genera of endophytic bacteria in Zanthoxylum. The most prevalent phyla, classes, orders, and genera were identified respectively as Proteobacteria, Gammaproteobacteria, Burkholderiales, and Pseudomonas. In this study, an endophytic growth-promoting bacterium was isolated and identified as sp. The results revealed that the bacterium exhibited robust nitrogen fixation, phosphorus solubilization, and effective siderophore production capabilities. The phosphate solubilization index (SI) was found to be (1.266 ± 0.0157). Following a 48-h incubation period in an inorganic phosphorus liquid medium (PKO), the concentration of auxin (IAA) and gibberellin (GA) reached their highest levels, at (138.145 ± 65.111) μg/mL and (805.74 ± 123.86) μg/mL, respectively. Moreover, the study showed that the endophytic bacteria markedly enhanced the germination potential and rate of sorghum seeds, and promoted significantly the growth of the tissue culture seedlings of Zanthoxylum.
花椒（Zanthoxylum）生长缓慢是导致其野生资源稀缺的重要因素。据报道，植物内生菌可通过提高植物养分吸收效率和调节植物激素来促进植物生长和次生代谢产物的合成。探索内生菌对花椒生长的促进作用具有重要意义。本研究应用高通量测序技术揭示了花椒中存在3个门、5个纲、7个目和11个属的内生细菌。最普遍的门、纲、目和属分别被鉴定为变形菌门、γ-变形菌纲、伯克霍尔德氏菌目和假单胞菌属。在本研究中，分离出一种促进生长的内生细菌并鉴定为sp。结果表明，该细菌具有强大的固氮、解磷和有效产生铁载体的能力。发现解磷指数（SI）为（1.266±0.0157）。在无机磷液体培养基（PKO）中培养48小时后，生长素（IAA）和赤霉素（GA）的浓度分别达到最高水平，即（138.145±65.111）μg/mL和（805.74±123.86）μg/mL。此外，研究表明，内生细菌显著提高了高粱种子的发芽势和发芽率，并显著促进了花椒组织培养苗的生长。
