Pominova D V, Ryabova A V, Skobeltsin A S, Markova I V, Romanishkin I D
PhD, Senior Researcher, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Associate Professor, Department 87 "Laser Micro-, Nano-, and Biotechnologies, Engineering Physics Institute for Biomedicine"; National Research Nuclear University MEPhI, 31 Kashirskoye Highway, Moscow, 115409, Russia.
Senior Researcher, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Associate Professor, Department 87 "Laser Micro-, Nano-, and Biotechnologies, Engineering Physics Institute for Biomedicine"; National Research Nuclear University MEPhI, 31 Kashirskoye Highway, Moscow, 115409, Russia.
Sovrem Tekhnologii Med. 2025;17(1):18-25. doi: 10.17691/stm2025.17.1.02. Epub 2025 Feb 28.
is to study the interaction of methylene blue (MB) with NADH, FADH coenzymes and lactate, and to evaluate a long-term effect of its intravenous or oral introduction on tumor metabolism .
The MB interaction with NADH, FADH coenzymes and lactate was studied using absorption spectrophotometry. A long-term effect of MB on tumor metabolism was investigated on a mice model of Ehrlich carcinoma. The effect of MB on tumor metabolism was assessed using time-resolved fluorescence microscopy based on the NADH fluorescence lifetime.
NADH has been established to be the main coenzyme with which MB interacts. The reduction of the lactate quantity is mediated by the shift of tumor metabolism as a result of MB interaction with the NADH. In the experiments , no noticeable tumor growth rate reduction was observed in the groups with intravenous MB introduction in comparison with the control. In the group receiving MB with drinking water, a decrease of the tumor growth rate, reduction of oxygenation level, and a1/a2 metabolic index were observed, which confirms the shift from glycolysis to oxidative phosphorylation.
The possibility of using MB for the tumor metabolism correction and growth rate reduction has been demonstrated, however, the time of therapy and MB concentration should be optimized to obtain more pronounced therapeutic effect.
研究亚甲蓝（MB）与烟酰胺腺嘌呤二核苷酸（NADH）、黄素腺嘌呤二核苷酸（FADH）辅酶及乳酸的相互作用，并评估其静脉或口服给药对肿瘤代谢的长期影响。
采用吸收分光光度法研究MB与NADH、FADH辅酶及乳酸的相互作用。在艾氏腹水癌小鼠模型上研究MB对肿瘤代谢的长期影响。基于NADH荧光寿命，采用时间分辨荧光显微镜评估MB对肿瘤代谢的影响。
已确定NADH是MB相互作用的主要辅酶。MB与NADH相互作用导致肿瘤代谢改变，进而介导乳酸量的减少。实验中，与对照组相比，静脉注射MB的组未观察到明显的肿瘤生长速率降低。在饮用含MB水的组中，观察到肿瘤生长速率降低、氧合水平降低以及a1/a2代谢指数降低，这证实了从糖酵解向氧化磷酸化的转变。
已证明使用MB校正肿瘤代谢和降低生长速率的可能性，然而，应优化治疗时间和MB浓度以获得更显著的治疗效果。
