Roomans G M, Wei X, Ceder O, Kollberg H
Ultrastruct Pathol. 1982 Jul-Sep;3(3):285-93. doi: 10.3109/01913128209016654.
The chronically reserpinized rat has been suggested as an animal model for cystic fibrosis. X-ray microanalysis of thick and thin cryosections was carried out to assess elemental redistribution in the submandibular glands and the pancreas of reserpinized rats at the cellular and subcellular level. In the submandibular gland of reserpinized rats, calcium and magnesium concentrations were significantly elevated. Mucus globules, secretory granules, and endoplasmic reticulum were the primary sites of the localization of excess calcium and magnesium. A significant potassium loss from the gland had occurred, particularly from the serous cells. Electron microscopy of conventionally prepared tissue showed marked swelling of the endoplasmic reticulum, especially in mucous cells. The elemental changes in the pancreatic acinar cells of reserpinized rats were reminiscent of elemental redistribution connected with cell death: increased levels of sodium, chlorine, and calcium and decreased levels of magnesium and potassium. Ultrastructural changes included swelling of the endoplasmic reticulum and obstruction of the acinar lumen. It is concluded tha elemental redistribution in chronically reserpinized rats presents interesting parallels with cystic fibrosis.
长期服用利血平的大鼠已被提议作为囊性纤维化的动物模型。对厚的和薄的冷冻切片进行X射线微分析，以在细胞和亚细胞水平评估利血平化大鼠下颌下腺和胰腺中的元素重新分布。在利血平化大鼠的下颌下腺中，钙和镁的浓度显著升高。黏液球、分泌颗粒和内质网是过量钙和镁的主要定位部位。腺体出现了显著的钾流失，尤其是浆液细胞中的钾。常规制备组织的电子显微镜检查显示内质网明显肿胀，尤其是在黏液细胞中。利血平化大鼠胰腺腺泡细胞中的元素变化使人联想到与细胞死亡相关的元素重新分布：钠、氯和钙水平升高，镁和钾水平降低。超微结构变化包括内质网肿胀和腺泡管腔阻塞。得出的结论是，长期服用利血平的大鼠中的元素重新分布与囊性纤维化存在有趣的相似之处。
