Curry F R
Fed Proc. 1985 Jul;44(10):2610-3.
The removal of plasma proteins from a vascular perfusate results in increased labeling of the endothelial cell (EC) vesicles and increased permeability of the capillary wall to water and solutes. The hypothesis that albumin forms part of a molecular filter composed of a network of fibrous molecules is evaluated. The fibrous network covers the EC surface and penetrates the intercellular junctions. Albumin may simply occupy space within the matrix to increase the resistance to water flow and increase exclusion and restriction to diffusion of solutes. Electrostatic interactions between positively charged sites on albumin and negatively charged fibers may also order the fibrous network into a more selective array. In the presence of albumin, the fibrous network would determine the selectivity of the capillary wall. An alternative hypothesis, that a selective pathway is formed when albumin is adsorbed to the walls of the wide portion of the slit, is inconsistent with the area required for the diffusion of small solutes between the endothelial cells. However, the geometry of intercellular channels may partially determine the selectivity of the capillary wall when the fiber matrix containing albumin is disrupted.
从血管灌注液中去除血浆蛋白会导致内皮细胞（EC）囊泡的标记增加，以及毛细血管壁对水和溶质的通透性增加。对白蛋白构成由纤维分子网络组成的分子过滤器一部分的假说进行了评估。纤维网络覆盖EC表面并穿透细胞间连接。白蛋白可能只是占据基质内的空间，以增加对水流的阻力，并增加对溶质扩散的排斥和限制。白蛋白上带正电的位点与带负电的纤维之间的静电相互作用也可能使纤维网络排列成更具选择性的阵列。在有白蛋白存在的情况下，纤维网络将决定毛细血管壁的选择性。另一种假说，即当白蛋白吸附到裂隙宽部的壁上时形成选择性通道，与小溶质在内皮细胞之间扩散所需的面积不一致。然而，当含有白蛋白的纤维基质被破坏时，细胞间通道的几何形状可能部分决定毛细血管壁的选择性。
