Schwartz S, Ellefson M
Gastroenterology. 1985 Jul;89(1):19-26. doi: 10.1016/0016-5085(85)90740-1.
Blood, meat, or fish, or any combination thereof, were ingested by 9 normal volunteers to permit studies of the contained hemes during total gastrointestinal transit. Quantitative analysis of ingested heme and of fecal heme and its degradation products was made possible by a new specific and extremely sensitive test, HemoQuant. The average fecal recovery of hemoglobin-heme from 10 to 36 ml of blood was 88%, as determined in 13 separate studies. All Hemoccult tests remained negative despite greater than 20-fold increases in fecal heme. Up to 83% of the blood heme was converted in the intestinal tract to porphyrins. These porphyrins are included in the HemoQuant, but not in Hemoccult or other leukodye assays. Negligible amounts of heme were found in fish and fowl, and their ingestion led to no significant increase in fecal heme. An average of only 25% of the heme in ingested meat was subsequently recovered in feces. Control fecal values represented an average of approximately 0.5 ml of blood per day. The recovery data obtained show that fecal HemoQuant results reliably reflect the total amount of blood hemoglobin that enters the gastrointestinal tract.
9名正常志愿者摄入血液、肉类或鱼类，或它们的任意组合，以便在整个胃肠道转运过程中研究其中所含的血红素。一种新的特异性且极其灵敏的检测方法——血红素定量检测法（HemoQuant），使得对摄入的血红素以及粪便中的血红素及其降解产物进行定量分析成为可能。在13项独立研究中确定，从10至36毫升血液中摄入的血红蛋白血红素的粪便平均回收率为88%。尽管粪便血红素增加了20多倍，但所有潜血试验仍为阴性。高达83%的血液血红素在肠道内转化为卟啉。这些卟啉包含在血红素定量检测法中，但不包含在潜血试验或其他白细胞染色检测中。在鱼类和禽类中发现的血红素量可忽略不计，摄入它们不会导致粪便血红素显著增加。摄入肉类中平均只有25%的血红素随后在粪便中被回收。对照粪便值平均每天约为0.5毫升血液。所获得的回收率数据表明，粪便血红素定量检测结果可靠地反映了进入胃肠道的血红蛋白总量。
