Krgović Nemanja, Stojković Dejan, Ivanov Marija, Dimitrijević Milena, Menković Nebojša, Ilić Milan, Jovanović Miloš S, Šavikin Katarina, Živković Jelena
Institute for Medicinal Plants Research "Dr. Josif Pančić", 11000 Belgrade, Serbia.
University of Belgrade, Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, 11000 Belgrade, Serbia.
Fitoterapia. 2025 Apr;182:106477. doi: 10.1016/j.fitote.2025.106477. Epub 2025 Mar 10.
This study aimed to perform chemical characterization of black raspberry seed oil (Rubus occidentalis L., Rosaceae) from Serbia in terms of fatty acids and tocols composition, total carotenoid content, as well as to investigate its antioxidant/antimicrobial activities and in vitro wound-healing potential. GC/MS analysis revealed that linoleic (39.30 %), α-linolenic (30.49 %) and oleic (18.94 %) acid were dominant fatty acids. HPLC analysis highlighted γ-tocopherol as the prevailing tocopherol isomer (166.80 mg/100 g). Spectrophotometric method determined a total carotenoid content of 1.20 mg/100 g. Appreciable antiradical activity (DPPH - IC 3.02 mg/mL; ABTS - IC 1.33 mg/mL) and a high level of reducing ability (FRAP value of 393.74 μmol Fe/100 g) were observed. Significant antibacterial activity against Salmonella Typhimurium, Escherichia coli and Bacillus cereus, in addition to antifungal activity against strains from Aspergillus and Trichoderma genera, was demonstrated. By cell viability assay, no cytotoxicity (IC > 401 μg/mL) was established on human keratinocytes (HaCaT cells). The wound-healing activity, evaluated by scratch assay, was found to be 2.41-fold higher in HaCaT cells treated with 100 μg/mL of black raspberry seed oil (41.77 %) than in non-treated cells (17.34 %). Taken together, black raspberry seed oil holds promising health potential.
本研究旨在对塞尔维亚的黑树莓籽油（悬钩子属西方品种，蔷薇科）进行脂肪酸和生育酚成分、总类胡萝卜素含量的化学表征，并研究其抗氧化/抗菌活性及体外伤口愈合潜力。气相色谱/质谱分析表明，亚油酸（39.30%）、α-亚麻酸（30.49%）和油酸（18.94%）是主要脂肪酸。高效液相色谱分析突出显示γ-生育酚是主要的生育酚异构体（166.80毫克/100克）。分光光度法测定总类胡萝卜素含量为1.20毫克/100克。观察到明显的抗自由基活性（DPPH - IC50为3.02毫克/毫升；ABTS - IC50为1.33毫克/毫升）和较高的还原能力（FRAP值为393.74微摩尔铁/100克）。证明了对鼠伤寒沙门氏菌、大肠杆菌和蜡样芽孢杆菌有显著抗菌活性，此外对曲霉属和木霉属菌株有抗真菌活性。通过细胞活力测定，在人角质形成细胞（HaCaT细胞）上未发现细胞毒性（IC50>401微克/毫升）。通过划痕试验评估，用100微克/毫升黑树莓籽油处理的HaCaT细胞的伤口愈合活性（41.77%）比未处理细胞（17.34%）高2.41倍。综上所述，黑树莓籽油具有良好的健康潜力。
