Al-Rajhi Aisha M H, Abdelghany Tarek M, Almuhayawi Mohammed S, Alruhaili Mohammed H, Saddiq Amna A, Baghdadi Afra M, Al Jaouni Soad K, Albasri Hibah M, Waznah Moayad S, Alraddadi Faisal A, Selim Samy
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11725, Egypt.
Sci Rep. 2024 Dec 11;14(1):30445. doi: 10.1038/s41598-024-81157-9.
Black seed has been applied for several decades to cure an extensive variety of illnesses and ailments. In this report, the chemical profile of both crude and ozonized black seed oil was assessed after the oil was exposed to 0 to 5 L/minute of ozone for four hours. The in vitro effects of black seed oil following being exposed to ozone including antimicrobial properties versus Bacillus cereus (ATCC11778), Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), Salmonella typhi (ATCC 6539), and Klebsiella pneumoniae (ATCC13883), Candida albicans (ATCC10221), and Aspergillus niger (ATCC16888). Besides, antioxidant effects, anti-inflammatory capacity, and antineoplastic function versus HCT cells were assessed. The chemical profile of ozonized black seed oil showed elevation of essential molecules of oil as well as presence of some characteristic molecules to both forms of oil. Besides, it could be noticed that exposing of oil to ozone improves its antimicrobial activity towards all tested microbes except for C. albicans. Both forms of oil showed no activity towards A. niger. Black seed oil exposed to ozone showed a promising antioxidant capacity with IC of 2.93 ± 0.2 µg/ml. A dramatic improvement in anti-inflammatory impact of ozonized oil as well as its antitumor capacity towards HCT cells could be seen in the laboratory outcomes. The current findings point to a novel method for enhancing some of the in vitro medicinal uses of black seed oil by exposing it to ozone.
黑种草籽已被应用数十年，用于治疗各种各样的疾病和不适。在本报告中，将黑种草籽油暴露于0至5升/分钟的臭氧中4小时后，评估了粗制和臭氧化黑种草籽油的化学特征。研究了黑种草籽油暴露于臭氧后的体外效应，包括对蜡状芽孢杆菌（ATCC11778）、金黄色葡萄球菌（ATCC6538）、大肠杆菌（ATCC8739）、伤寒沙门氏菌（ATCC 6539）、肺炎克雷伯菌（ATCC13883）、白色念珠菌（ATCC10221）和黑曲霉（ATCC16888）的抗菌性能。此外，还评估了其对HCT细胞的抗氧化作用、抗炎能力和抗肿瘤功能。臭氧化黑种草籽油的化学特征表明，油中的必需分子含量增加，并且两种形式的油都存在一些特征性分子。此外，可以注意到，将油暴露于臭氧中可提高其对除白色念珠菌外的所有测试微生物的抗菌活性。两种形式的油对黑曲霉均无活性。暴露于臭氧的黑种草籽油显示出有前景的抗氧化能力，IC50为2.93±0.2微克/毫升。在实验室结果中可以看到，臭氧化油的抗炎作用及其对HCT细胞的抗肿瘤能力有显著改善。目前的研究结果指出了一种通过将黑种草籽油暴露于臭氧来增强其某些体外药用价值的新方法。
