State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization(MOA), Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Int J Mol Sci. 2020 Jan 18;21(2):634. doi: 10.3390/ijms21020634.
Banana (, AAA group) is a representative climacteric fruit with essential nutrients and pleasant flavors. Control of its ripening determines both the fruit quality and the shelf life. NAC (NAM, ATAF, CUC2) proteins, as one of the largest superfamilies of transcription factors, play crucial roles in various functions, especially developmental processes. Thus, it is important to conduct a comprehensive identification and characterization of the NAC transcription factor family at the genomic level in . In this article, a total of 181 banana NAC genes were identified. Phylogenetic analysis indicated that NAC genes in , Arabidopsis, and rice were clustered into 18 groups (S1-S18), and MCScanX analysis disclosed that the evolution of MaNAC genes was promoted by segmental duplication events. Expression patterns of NAC genes during banana fruit ripening induced by ethylene were investigated using RNA-Seq data, and 10 MaNAC genes were identified as related to fruit ripening. A subcellular localization assay of selected MaNACs revealed that they were all localized to the nucleus. These results lay a good foundation for the investigation of NAC genes in banana toward the biological functions and evolution.
香蕉（AAA 组）是一种具有重要营养和宜人风味的代表性跃变型果实。其成熟度的控制决定了果实的品质和货架期。NAC（NAM、ATAF、CUC2）蛋白作为最大的转录因子超家族之一，在各种功能中发挥着关键作用，尤其是在发育过程中。因此，在基因组水平上对香蕉 NAC 转录因子家族进行全面鉴定和特征描述是非常重要的。在本文中，共鉴定到 181 个香蕉 NAC 基因。系统发育分析表明，香蕉、拟南芥和水稻中的 NAC 基因聚类为 18 个组（S1-S18），MCScanX 分析揭示了 MaNAC 基因的进化是由片段复制事件推动的。利用 RNA-Seq 数据研究了乙烯诱导香蕉果实成熟过程中 NAC 基因的表达模式，鉴定到 10 个与果实成熟相关的 MaNAC 基因。对选定的 MaNAC 进行亚细胞定位分析表明，它们均定位于细胞核。这些结果为研究香蕉 NAC 基因的生物学功能和进化奠定了良好的基础。
