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川西樱桃(Prunus serrula Franch)基因组的染色体水平组装

Chromosome-level assembly of Prunus serrula Franch genome.

作者信息

Zuo Hao, Liu Shengjun, Tan Lei, Huang Yue, Li Yuanrong, Gesang Pingcuo, Hong Ying, Deng Xiuxin, Wang Xia, Xu Qiang, Jiao Wen-Biao, Zeng Xiuli

机构信息

Qinghai-Tibet Plateau Fruit Trees Scientific Observation Test Station (Ministry of Agriculture and Rural Affairs), Lhasa, Tibet, 850032, China.

National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Wuhan, Hubei, 430070, P.R. China.

出版信息

Sci Data. 2025 Mar 24;12(1):494. doi: 10.1038/s41597-025-04810-6.

Abstract

P. serrula is widely distributed in Yunnan, Xizang, and Sichuan, and usually grows at high altitudes between 2,600 and 3,900 meters above sea level. In this study, we obtained a high-quality chromosome-level assembly genome of P. serrula using Illumina sequencing, Oxford Nanopore ultra-long sequencing, and Hi-C technology. The genome was 284.5 Mb in length, with a scaffold N50 of 32.4 Mb and 91.9% of the assembly anchored onto 8 pseudochromosomes. BUSCO completeness value of 98.5% demonstrated a high completed genome, and a total of 35,151 protein-coding genes and 47,340 transcripts were annotated. Overall, this genome delivers valuable genetic resources for further phylogenomic studies and provides insights into the genetic architectures underlying high-altitude adaptation.

摘要

细叶鲜卑花广泛分布于云南、西藏和四川,通常生长在海拔2600至3900米的高海拔地区。在本研究中,我们利用Illumina测序、牛津纳米孔超长测序和Hi-C技术获得了高质量的细叶鲜卑花染色体水平的组装基因组。该基因组长度为284.5 Mb,支架N50为32.4 Mb,91.9%的组装序列锚定在8条假染色体上。BUSCO完整性值为98.5%,表明基因组完整性高,共注释了35151个蛋白质编码基因和47340个转录本。总体而言,该基因组为进一步的系统发育基因组学研究提供了宝贵的遗传资源,并为高海拔适应的遗传结构提供了见解。

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