Li Shufang, Wang Hairong, Li Boyang, Lu Henan, Zhao Jianxin, Gao Aiwu, An Yawen, Yang Jinli, Ma Tian
Animal Nutrition and Feed Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Food Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Animals (Basel). 2025 Mar 5;15(5):749. doi: 10.3390/ani15050749.
Feeding HC diets has been found to induce metabolic dysregulation in the colon. However, the mechanisms by which changes in colonic flora and metabolites damage the colonic epithelium are poorly studied. Therefore, the present experiment used a multi-omics technique to investigate the mechanism of colonic injury induced by high-concentrate diets in lambs. Twelve male Dumont lambs were randomly split into two groups: a low-concentrate diet (LC = concentrate/forage = 30:70) group and a high-concentrate diet (HC = concentrate/forage = 70:30) group. The results showed that the HC group presented significantly increased lipopolysaccharide (LPS) concentrations in the colonic epithelium and significantly decreased serum total cholesterol (TC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels ( < 0.05), which led to cavities and inflammatory cell infiltration in the colonic epithelium. The HC group had significantly lower pH and less VFAs in colon contents, as well as a significantly increased abundance of bacteria of the genera , , , , , , , , and compared to the LC diet group. These microorganisms significantly increased the level of metabolites of cholic acid, chenodeoxycholic acid, LysoPA (P-16:0/0:0), methapyrilene, and fusaric acid. A transcriptome analysis showed that cytokine-cytokine receptor interaction, glutathione metabolism, and the peroxisome signaling pathway were downregulated in the colon epithelium of the lambs fed the HC diet. Therefore, the HC diet caused epithelial inflammation and oxidative damage by affecting the interaction between the microbial flora of the colon and metabolites and the host epithelium, which eventually disrupted colon homeostasis and had a negative impact on sheep health.
研究发现，给羔羊饲喂高浓缩日粮会导致结肠代谢失调。然而，关于结肠菌群和代谢产物的变化如何损害结肠上皮的机制，目前研究较少。因此，本实验采用多组学技术研究高浓缩日粮诱导羔羊结肠损伤的机制。将12只雄性杜蒙羔羊随机分为两组：低浓缩日粮（LC = 精料/草料 = 30:70）组和高浓缩日粮（HC = 精料/草料 = 70:30）组。结果显示，HC组结肠上皮中脂多糖（LPS）浓度显著升高，血清总胆固醇（TC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平显著降低（P<0.05），导致结肠上皮出现空洞和炎性细胞浸润。与LC日粮组相比，HC组结肠内容物的pH值显著降低，挥发性脂肪酸（VFA）含量减少， 、 、 、 、 、 、 、 和 属细菌的丰度显著增加。这些微生物显著提高了胆酸、鹅去氧胆酸、溶血磷脂酸（P-16:0/0:0）、甲氧苄二氨嘧啶和镰刀菌酸的代谢产物水平。转录组分析表明，在饲喂HC日粮的羔羊结肠上皮中，细胞因子-细胞因子受体相互作用、谷胱甘肽代谢和过氧化物酶体信号通路下调。因此，HC日粮通过影响结肠微生物菌群与代谢产物以及宿主上皮之间的相互作用，导致上皮炎症和氧化损伤，最终破坏结肠内环境稳定，对绵羊健康产生负面影响。
