Cetin Fatma Sude, Avci Tubanur, Uygur Emre, Ilhan Elif, Kaya Elif, Tinaz Gulgun Bosgelmez, Duta Liviu, Dogan Canan, Gunduz Oguzhan
Center for Nanotechnology and Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey.
Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul 34722, Turkey.
Polymers (Basel). 2025 Mar 5;17(5):693. doi: 10.3390/polym17050693.
This study introduces novel chitosan (CS) and polyethylene oxide (PEO) copolymers reinforced with bacterial cellulose (BC) to fabricate nanofibers using the electrospinning method. SEM analysis confirmed uniform nanofiber formation, with CS/PEO/BC nanofibers (240 nm) exhibiting a larger diameter than CS/PEO ones (190 nm). FTIR spectroscopy confirmed BC integration, while Differential scanning calorimetry analysis indicated minimal impact on glass transition temperature. Notably, as compared to CS/PEO nanofibers, the CS/PEO/BC ones demonstrated superior swelling capacity, accelerated biodegradation, and enhanced mechanical (i.e., tensile) properties, with maximum stress and strain values of ~3.41 MPa and ~0.01% vs. ~2.14 MPa and ~0.01%. Antimicrobial assays confirmed activity against bacterial strains, and biocompatibility tests showed high cell viability at day seven (99.26% for CS/PEO/BC nanofibers). These findings highlight the potential of CS/PEO/BC nanofibers as promising candidates for tissue engineering, offering improved strength, biodegradability, and antimicrobial properties.
本研究引入了用细菌纤维素（BC）增强的新型壳聚糖（CS）和聚环氧乙烷（PEO）共聚物，采用静电纺丝法制备纳米纤维。扫描电子显微镜（SEM）分析证实形成了均匀的纳米纤维，CS/PEO/BC纳米纤维（约240纳米）的直径比CS/PEO纳米纤维（约190纳米）更大。傅里叶变换红外光谱（FTIR）证实了BC的整合，而差示扫描量热法分析表明对玻璃化转变温度的影响最小。值得注意的是，与CS/PEO纳米纤维相比，CS/PEO/BC纳米纤维表现出更高的溶胀能力、更快的生物降解速度和更强的机械（即拉伸）性能，其最大应力和应变值分别约为3.41兆帕和0.01%，而CS/PEO纳米纤维分别约为2.14兆帕和0.01%。抗菌试验证实了对细菌菌株的活性，生物相容性测试表明在第7天细胞活力很高（CS/PEO/BC纳米纤维为99.26%）。这些发现突出了CS/PEO/BC纳米纤维作为组织工程有前景候选材料的潜力，具有改善的强度、生物降解性和抗菌性能。
