Salami Chefikou, Mbakidi Jean-Pierre, Audonnet Sandra, Brassart-Pasco Sylvie, Bouquillon Sandrine
Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, Université de Reims Champagne-Ardenne, Boîte n° 44, B.P. 1039, 51687 Reims, France.
URCATech, URCACyt, Université de Reims Champagne-Ardenne, 51 Rue Cognacq Jay CS30018, 51095 Reims, France.
Molecules. 2025 Mar 6;30(5):1180. doi: 10.3390/molecules30051180.
Six biobased ionic liquids were prepared from saturated fatty acids (octanoic, decanoic and dodecanoic acids) and choline with yields up to 90% following procedures respecting green chemistry principles. These ionic liquids were fully characterized (NMR, IR, elemental analysis, viscosimetry and TGA) and used as extraction solvents for bioactive compounds (curcuminoids and carvacrol) using classical conditions, and the ionic liquids were able to be recovered after five runs without loss of activity. The ionic liquid containing a C12 carbon chain was the best extracting solvent, extracting 95% of the total curcuminoids contained in turmeric and 69% of the total carvacrol contained in oregano, which are higher yields compared to the extraction procedures described in the literature. As C12 ionic liquids were more cytotoxic than C8 ones, the biological activity of the curcuminoids extracted with C8 ionic liquids was evaluated on a MIAPaCa-2, a pancreatic adenocarcinoma cell line for which antitumor activity of curcuminoids had previously been reported. Compared to the cytotoxicity of the commercially available extract, the cytotoxic activity of the extracts was slightly weaker.
按照绿色化学原则，由饱和脂肪酸（辛酸、癸酸和月桂酸）与胆碱制备了六种生物基离子液体，产率高达90%。对这些离子液体进行了全面表征（核磁共振、红外光谱、元素分析、粘度测定和热重分析），并在经典条件下用作生物活性化合物（姜黄素类化合物和香芹酚）的萃取溶剂，且经过五次循环后离子液体能够回收，活性未损失。含C12碳链的离子液体是最佳萃取溶剂，能萃取出姜黄中95%的总姜黄素类化合物以及牛至中69%的总香芹酚，与文献中描述的萃取方法相比，产率更高。由于C12离子液体比C8离子液体的细胞毒性更强，因此对用C8离子液体萃取的姜黄素类化合物在MIAPaCa - 2（一种先前已报道姜黄素类化合物具有抗肿瘤活性的胰腺腺癌细胞系）上的生物活性进行了评估。与市售提取物的细胞毒性相比，这些提取物的细胞毒性活性稍弱。
