Costa Christopher J, Prescott Stephanie, Fourie Nicolaas H, Abey Sarah K, Sherwin LeeAnne B, Rahim-Williams Bridgett, Joseph Paule V, Posada-Quintero Hugo, Hoffman Rebecca K, Henderson Wendy A
Department of Medicine, UConn Health, 263 Farmington Ave, Farmington, CT 06030, USA.
Inova Health Services, L.J. Murphy Children's Hospital, 3300 Gallows Rd, Falls Church, VA 22042, USA.
Nutrients. 2025 Mar 6;17(5):921. doi: 10.3390/nu17050921.
Chronic visceral hypersensitivity is associated with an overstressed pain response to noxious stimuli (hyperalgesia). Microbiota are active modulators of host biology and are implicated in the etiology of visceral hypersensitivity.
we studied the association between the circulating mRNA transcriptome, the intensity of induced visceral pain (IVP), and variation in the oral microbiome among participants with and without baseline visceral hypersensitivity.
Transcriptomic profiles and microbial abundance were correlated with IVP intensity. Host mRNA and microbes associated with IVP were explored, linking variation in the microbiome to host RNA biology.
259 OTUs were found to be associated with IVP through correlation to differential expression of 471 genes in molecular pathways related to inflammation and neural mechanisms, including Rho and PI3K/AKT pathways. The bacterial families Lachnospiraceae, Prevotellaceae, and Veillonellaceae showed the highest degree of association. Oral microbial profiles with reduced diversity were characteristic of participants with visceral hypersensitivity.
Our results suggest that the oral microbiome may be involved in systemic immune and inflammatory effects and play a role in nervous system and stem cell pathways. The interactions between visceral hypersensitivity, differentially expressed molecular pathways, and microbiota described here provide a framework for further work exploring the relationship between host and microbiome.
慢性内脏超敏反应与对有害刺激的过度应激性疼痛反应（痛觉过敏）相关。微生物群是宿主生物学的活跃调节因子，并与内脏超敏反应的病因有关。
我们研究了有或无基线内脏超敏反应的参与者的循环mRNA转录组、诱发内脏痛（IVP）强度和口腔微生物群变化之间的关联。
将转录组谱和微生物丰度与IVP强度相关联。探索与IVP相关的宿主mRNA和微生物，将微生物群的变化与宿主RNA生物学联系起来。
通过与炎症和神经机制相关分子途径中471个基因的差异表达相关，发现259个操作分类单元（OTU）与IVP相关，包括Rho和PI3K/AKT途径。毛螺菌科、普雷沃氏菌科和韦荣氏菌科显示出最高程度的关联。内脏超敏反应参与者的特征是口腔微生物谱多样性降低。
我们的结果表明，口腔微生物群可能参与全身免疫和炎症效应，并在神经系统和干细胞途径中发挥作用。此处描述的内脏超敏反应、差异表达的分子途径和微生物群之间的相互作用为进一步探索宿主与微生物群之间的关系提供了框架。
