Tang Binbin, Hu Yunlian, Chen Jianhui, Su Chengxia, Zhang Qian, Huang Chaoqun
Second Outpatient Department, Tongde Hospital of Zhejiang Province, Hangzhou, China.
Clinical College of Traditional Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, China.
Heliyon. 2023 Jan 20;9(1):e13114. doi: 10.1016/j.heliyon.2023.e13114. eCollection 2023 Jan.
This study aimed at investigating the characteristics and correlation between oral (tongue coating) and fecal microbiota in patients with diarrheal irritable bowel syndrome (IBS-D).
Fifty-two IBS-D patients were chosen, with ten healthy volunteers serving as the normal control group. Tongue coating samples and fecal samples of subjects were sequenced for the 16S rRNA gene (V4-V5). Bioinformatics analysis was done on the test data to investigate oral and fecal microbiota composition characteristics in IBS-D patients.
The microbial richness of tongue coating in IBS-D group was lower than that in the normal control group (P < 0.05). The beta diversity of tongue coating microbiota and fecal microbiota was significantly different in the IBS-D group compared to the normal control group (P < 0.05). ( and ), , , , and were considerably high in number the tongue coating samples of the IBS-D group in comparison to the normal control group. Similarly, the fecal samples from the IBS-D group were significantly enriched in , ( and ), , , , and compared to the normal control group.
The oral and fecal microbiotas of IBS-D patients differ from those of the control group; hence studying IBS-D from the perspective of the oral-gut microbiome axis is an interesting research avenue.
本研究旨在调查腹泻型肠易激综合征（IBS-D）患者口腔（舌苔）和粪便微生物群的特征及相关性。
选取52例IBS-D患者，10名健康志愿者作为正常对照组。对受试者的舌苔样本和粪便样本进行16S rRNA基因（V4-V5）测序。对测试数据进行生物信息学分析，以研究IBS-D患者口腔和粪便微生物群的组成特征。
IBS-D组舌苔的微生物丰富度低于正常对照组（P<0.05）。与正常对照组相比，IBS-D组舌苔微生物群和粪便微生物群的β多样性有显著差异（P<0.05）。与正常对照组相比，IBS-D组舌苔样本中的（和）、、、、数量相当多。同样，与正常对照组相比，IBS-D组的粪便样本在、（和）、、、中显著富集。
IBS-D患者的口腔和粪便微生物群与对照组不同；因此从口腔-肠道微生物群轴的角度研究IBS-D是一个有趣的研究途径。
