Priscott P K, Ford J R
In Vitro Cell Dev Biol. 1985 Feb;21(2):88-92. doi: 10.1007/BF02620948.
A culture model is described for the study of acetaldehyde (AcH) metabolism by explanted postimplantation rat and mouse conceptuses. The ability of 12-d rat and 10-d mouse embryos to metabolise AcH was demonstrated. The elimination rate for the 12-d rat conceptus using an initial AcH concentration of 1 mM in the medium was found to be 1.8 nmol/mg per minute. When the conceptus was divided into embryonic and extraembryonic tissue, the rates were 1.6 and 2.2 nmol/mg per minute, respectively. When the AcH concentration was reduced to 50 microM the rate was 0.095 nmol/mg per minute. The results provide further evidence for a functional barrier that prevents AcH entry to the embryo. A comparative experiment using CBA/beige mouse conceptuses showed that AcH elimination characteristics may be qualitatively similar to those in rat embryos, but that the estimated elimination rate of 0.8 nmol/mg per minute was less than half that of the rat. Thus the "metabolic barrier" may be less efficient in the mouse. This may be important in view of the greater sensitivity of the mouse to ethanol embryotoxicity.
本文描述了一种用于研究植入后大鼠和小鼠胚胎对乙醛（AcH）代谢的培养模型。研究证明了12日龄大鼠胚胎和10日龄小鼠胚胎具有代谢AcH的能力。在培养基中初始AcH浓度为1 mM时，12日龄大鼠胚胎的消除速率为每分钟1.8 nmol/mg。当将胚胎分为胚胎组织和胚外组织时，速率分别为每分钟1.6 nmol/mg和2.2 nmol/mg。当AcH浓度降至50 microM时，速率为每分钟0.095 nmol/mg。这些结果为阻止AcH进入胚胎的功能屏障提供了进一步的证据。使用CBA/米色小鼠胚胎进行的对比实验表明，AcH的消除特性在质量上可能与大鼠胚胎相似，但估计的消除速率为每分钟0.8 nmol/mg，不到大鼠的一半。因此，小鼠中的“代谢屏障”可能效率较低。鉴于小鼠对乙醇胚胎毒性更为敏感，这一点可能很重要。
