Kinouchi T, Kotake T, Mori Y, Abe T
In Vitro Cell Dev Biol. 1985 Apr;21(4):195-9. doi: 10.1007/BF02620929.
A cell line, designated as OS-RC-2, has been established from a renal cell carcinoma in a 52-yr-old Japanese male patient and maintained for 23 mo. through 60 in vitro passages. The OS-RC-2 formed monolayers of polygonal epithelial cells and lacked contact inhibition. Doubling time of cells was about 60 h at the 30th passage. Electron microscopic findings indicated numerous long microvilli on the cell surface and many glycogen granules in the cytoplasm of this cell line, which are characteristic structures of renal cell carcinoma. Chromosomal analysis revealed that a small portion of this cell line had a hypodiploid modal number of 40 and a large portion had a hypotetraploid modal number of 75. The characteristics of the karyotype were one detected marker chromosome and the translocation between the Chromosomes 2 and 13. Cell line OS-RC-2 was serially transplantable into nude mice, and histopathological findings of heterotransplanted tumor showed a close similarity to those of the original tumor. Histocompatibility antigens of OS-RC-2 were HLA-A9, Bw52, which were identical to those of the peripheral blood lymphocytes of the patient.
一种名为OS-RC-2的细胞系，是从一名52岁日本男性患者的肾细胞癌中建立的，并通过60次体外传代培养了23个月。OS-RC-2形成多边形上皮细胞单层，且缺乏接触抑制。在第30代时，细胞的倍增时间约为60小时。电子显微镜检查结果显示，该细胞系的细胞表面有许多长微绒毛，细胞质中有许多糖原颗粒，这些都是肾细胞癌的特征性结构。染色体分析表明，该细胞系的一小部分具有40的亚二倍体众数，大部分具有75的亚四倍体众数。核型特征为一条检测到的标记染色体以及2号和13号染色体之间的易位。细胞系OS-RC-2可连续移植到裸鼠体内，异种移植肿瘤的组织病理学结果与原发肿瘤非常相似。OS-RC-2的组织相容性抗原为HLA-A9、Bw52，与患者外周血淋巴细胞的抗原相同。
