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从食用菌六棱羊肚菌中分离出的一种β-内病毒的特性研究

Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata.

作者信息

Lyu Ruiling, Chen Junlin, Tang Qing, Hai Du, Wu Tun, Xiao Huagang, Xie Jiatao, Xiao Yang

机构信息

State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Hubei Key Laboratory for the Improvement of Economic Forest Germplasm and Comprehensive Utilization of Resources, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, College of Biology and Agricultural Resource, Huanggang Normal University, Huanggang, Hubei, 438000, P.R. China.

出版信息

Arch Virol. 2025 Mar 14;170(4):78. doi: 10.1007/s00705-025-06264-y.

DOI:10.1007/s00705-025-06264-y
PMID:40085276
Abstract

A positive-sense single-stranded RNA mycovirus was isolated from Morchella sextelata strain ZY-1 and designated as "Morchella sextelata endornavirus 1" (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus Betaendornavirus in the family Endornaviridae.

摘要

从六棱羊肚菌菌株ZY-1中分离出一种正义单链RNA真菌病毒,并将其命名为“六棱羊肚菌内病毒1”(MsEV1)。发现MsEV1的完整基因组长度为16589个核苷酸(nt),并包含一个单一的大开放阅读框(ORF),该阅读框在一个由5477个氨基酸组成的多蛋白中编码病毒蛋白PRK00409、甲基转移酶、DEXDc、解旋酶和RNA依赖的RNA聚合酶(RdRP)。基于RdRP核苷酸序列的系统发育分析表明,这种内病毒是内病毒科β内病毒属的成员。

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