Song Ha-Yeon, Choi Hyo-Jin, Jeong Hansaem, Choi Dahye, Kim Dae-Hyuk, Kim Jung-Mi
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju 54896, Korea.
Mycobiology. 2016 Dec;44(4):283-290. doi: 10.5941/MYCO.2016.44.4.283. Epub 2016 Dec 31.
A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free strains with a virus-infected strain, isogenic virus-cured strains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infected strain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeastmalt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth of .
在商业化瓶栽食用平菇菌株ASI2792的畸形子实体中检测到一种双链RNA（dsRNA）真菌病毒。克隆了ASI2792真菌病毒（PoV-ASI2792）的部分RNA依赖RNA聚合酶（RdRp）基因，cDNA序列比对显示该序列与在该菌株中发现的一种已知PoSV的RdRp基因相同。为了通过比较同基因无病毒菌株和病毒感染菌株来研究PoV-ASI2792感染的症状，采用菌丝体片段化法获得了同基因病毒清除菌株。在dsRNA特异性病毒纯化后，通过凝胶电泳验证病毒的缺失，并使用PoV-ASI2792的部分RdRp cDNA进行Northern印迹分析。将感染PoV-ASI2792真菌病毒的菌株与三种无病毒同基因菌株在10种不同培养基上的生长速率和菌丝干重进行了比较。病毒清除菌株在所有实验培养基上均表现出明显更高的菌丝生长速率和干重，在蘑菇完全培养基（MCM）和滨田培养基上菌丝生长速率至少比病毒感染菌株高2.2倍，在MCM和酵母麦芽葡萄糖琼脂培养基上菌丝干重比病毒感染菌株高2.7倍。这些结果表明，PoV真菌病毒的感染对平菇的营养生长有有害影响。
