Clayton L, Black C M, Lloyd C W
J Cell Biol. 1985 Jul;101(1):319-24. doi: 10.1083/jcb.101.1.319.
Human scleroderma serum 5051, which is known to recognize the amorphous pericentriolar microtubule organizing center material of a variety of vertebrate cells, was found to immunostain spindle poles of meristematic higher plants from pre-prophase to late anaphase. Subsequently, during cytokinesis, staining was redistributed around the reforming telophase nuclei, but was not evident in the cytokinetic phragmoplast. At the transition between telophase and interphase, before the typical cortical interphase microtubule array was established, short microtubules radiated from the nucleus and in such cells the material recognized by 5051 was located around the daughter nuclei and not the cortex. These observations have led us to propose that the perinuclear region, or the nuclear surface, may function as a nucleation center for both spindle and interphase microtubules in higher plant cells.
人硬皮病血清5051已知可识别多种脊椎动物细胞中无定形的中心粒周围微管组织中心物质，研究发现它能对处于前期到后期的高等植物分生组织的纺锤体极进行免疫染色。随后，在胞质分裂期间，染色质重新分布在重新形成的末期细胞核周围，但在胞质分裂的成膜体中不明显。在末期和间期的过渡阶段，在典型的皮层间期微管阵列形成之前，短微管从细胞核中放射出来，在这些细胞中，5051识别的物质位于子细胞核周围而非皮层。这些观察结果使我们提出，核周区域或核表面可能作为高等植物细胞中纺锤体和间期微管的成核中心发挥作用。
