Williams R K, Goridis C, Akeson R
J Cell Biol. 1985 Jul;101(1):36-42. doi: 10.1083/jcb.101.1.36.
The neural cell adhesion molecules, or N-CAMs, are a group of structurally and immunologically related glycoproteins found in vertebrate neural tissues. Adult brain N-CAMs have apparent molecular weights of 180,000, 140,000, and 120,000. In this article we identify, using monoclonal antibody (Mab) 3G6.41, an immunologically distinct adult rat N-CAM form and show that this form is selectively expressed by some clonal neural cell lines. Consecutive immunoprecipitation experiments indicate that rabbit anti-N-CAM can remove from solubilized cerebellar neuron primary cultures all 180,000- and 140,000-mol-wt N-CAM molecules that react with Mab 3G6.41. However Mab 3G6.41 cannot remove all N-CAM molecules that react with rabbit anti-N-CAM. Rabbit anti-N-CAM binds to and immunoprecipitates N-CAM forms from the rat neuronal cell lines B35, B65, and B104, the glial lines B12 and C6, and L6 myoblasts. Mab 3G6.41 does not bind to or immunoprecipitate N-CAM from the B12 and B65 lines but does react with the other four lines by both criteria. Many cells in primary cultures of postnatal rat that express glial fibrillary acidic protein also bind Mab 3G6.41. Thus a unique form of rat N-CAM recognized by Mab 3G6.41 is found on some but not all neuronal, glial, and muscle cells.
神经细胞黏附分子，即N-CAMs，是在脊椎动物神经组织中发现的一组结构和免疫相关的糖蛋白。成年大脑中的N-CAMs的表观分子量分别为180,000、140,000和120,000。在本文中，我们使用单克隆抗体（Mab）3G6.41鉴定出一种免疫上不同的成年大鼠N-CAM形式，并表明这种形式在一些克隆神经细胞系中选择性表达。连续免疫沉淀实验表明，兔抗N-CAM可以从溶解的小脑神经元原代培养物中去除所有与Mab 3G6.41反应的180,000和140,000分子量的N-CAM分子。然而，Mab 3G6.41不能去除所有与兔抗N-CAM反应的N-CAM分子。兔抗N-CAM能结合并免疫沉淀大鼠神经元细胞系B35、B65和B104、神经胶质细胞系B12和C6以及L6成肌细胞中的N-CAM形式。Mab 3G6.41不与B12和B65细胞系中的N-CAM结合或免疫沉淀，但根据这两个标准，它确实与其他四个细胞系反应。出生后大鼠原代培养物中许多表达胶质纤维酸性蛋白的细胞也能结合Mab 3G6.41。因此，在一些但不是所有的神经元、神经胶质细胞和肌肉细胞上发现了一种被Mab 3G6.41识别的独特形式的大鼠N-CAM。
