Man Yuanzhuo, Li Weichi, Xu Xin, Lu Xiaonan, Li Dandan, Yao Lunguang, Ji Jun, Bi Yingzuo, Xie Qingmei
Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering, and Technology Center of Health Products for Livestock and Poultry, Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Nanyang Normal University, Nanyang 473061, PR China.
Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering, and Technology Center of Health Products for Livestock and Poultry, Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Nanyang Normal University, Nanyang 473061, PR China.
Poult Sci. 2025 May;104(5):104984. doi: 10.1016/j.psj.2025.104984. Epub 2025 Mar 5.
Duck Associated Chapparvovirus (DaChPV) is a newly discovered virus within the Chaphamparvovirus genus, and first identified from Canadian wild ducks in 2021. In this study, DaChPV DNA was detected in 14 out of 137 tissues samples collected from diarrhea ducks across various provinces in China. Subsequently, eight complete genome sequences were amplified using overlapping primers and sequenced. Comparative analysis revealed that the amino acid (aa) sequences of NS1 and VP1 from these eight DaChPV strains shared identity with reference strains, ranging from 81.43 % to 98.81 % for NS1 and 76.95 % to 98.39 % for VP1, respectively. Phylogenetic analysis of the genome sequences showed that the newly identified DaChPVs and reference DaChPV strains formed an independent cluster, indicating a close genetic relationship. The strains of AH2301, HN2201 and HN2301 identified in this study were classified as belonging to Duck Associated Chaphamapavovirus 1. In contrast, AH2401, HN2401, and SD2301 were grouped with Duck Associated Chaphamapavovirus 2. The remaining two strains, HeB2201 and HeB2401, may represent variant strains that cluster independently, which is further supported by the evolutionary tree results of VP1 and NS1. Additionally, inter-type recombinations were predicted for these DaChPV strains. The contained multiple specific mutation sites, including 63, 76, 78, 303, and 305 that located on the predicted antigenic epitopes. This research firstly determined the evolutionary trends of DaChPV in China, offering valuable insights for understanding of spread, evolution, and molecular epidemiology of DaChPV on a global scale.
鸭相关细小病毒（DaChPV）是细小病毒属中一种新发现的病毒，于2021年首次从加拿大野鸭中鉴定出来。在本研究中，从中国各省腹泻鸭采集的137份组织样本中，有14份检测到DaChPV DNA。随后，使用重叠引物扩增并测序了8个完整的基因组序列。比较分析表明，这8个DaChPV毒株的NS1和VP1氨基酸（aa）序列与参考毒株具有同源性，NS1的同源性为81.43%至98.81%，VP1的同源性为76.95%至98.39%。基因组序列的系统发育分析表明，新鉴定的DaChPV与参考DaChPV毒株形成一个独立的聚类，表明它们具有密切的遗传关系。本研究中鉴定的AH2301、HN2201和HN2301毒株被归类为鸭相关查帕马细小病毒1。相比之下，AH2401、HN2401和SD2301则与鸭相关查帕马细小病毒2归为一组。其余两个毒株HeB2201和HeB2401可能代表独立聚类的变异毒株，VP1和NS1的进化树结果进一步支持了这一点。此外，预测这些DaChPV毒株存在型间重组。它们包含多个特定的突变位点，包括位于预测抗原表位上的63、76、78、303和305位点。本研究首次确定了DaChPV在中国的进化趋势，为在全球范围内了解DaChPV的传播、进化和分子流行病学提供了有价值的见解。
