气相色谱法用于艰难梭菌相关疾病快速诊断的评估
Evaluation of gas-liquid chromatography for the rapid diagnosis of Clostridium difficile associated disease.
作者信息
Gianfrilli P, Pantosti A, Luzzi I
出版信息
J Clin Pathol. 1985 Jun;38(6):690-3. doi: 10.1136/jcp.38.6.690.
Abstract
Direct gas-liquid chromatography of faecal specimens with isocaproic acid as a marker was used for the rapid diagnosis of Clostridium difficile associated diarrhoeal diseases. Ninety stools were examined and results were compared with conventional culture on selective medium and cytotoxin assay in tissue culture. Using a combined analysis of isocaproic acid and butyric acid peak heights we defined three categories: positive, negative, and indeterminate. When the indeterminate group was excluded, the positive and negative predictive values of gas-liquid chromatography analysis were 86.9% and 85% respectively compared with culture and 71.4% and 95% respectively compared with cytotoxin assay.
摘要
以异己酸作为标志物，采用粪便标本直接气液色谱法对艰难梭菌相关性腹泻疾病进行快速诊断。共检查了90份粪便样本，并将结果与在选择性培养基上的传统培养法以及组织培养中的细胞毒素检测法进行比较。通过对异己酸和丁酸峰高进行联合分析，我们定义了三类结果：阳性、阴性和不确定。排除不确定组后，气液色谱分析与培养法相比的阳性预测值和阴性预测值分别为86.9%和85%，与细胞毒素检测法相比分别为71.4%和95%。
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