人粪便中艰难梭菌毒素的快速检测
Rapid detection of Clostridium difficile toxin in human feces.
作者信息
Ryan R W, Kwasnik I, Tilton R C
出版信息
J Clin Microbiol. 1980 Dec;12(6):776-9. doi: 10.1128/jcm.12.6.776-779.1980.
Abstract
Fifty fecal specimens were tested by three methods, bacterial isolation, counterimmunoelectrophoresis, and tissue culture, for Clostridium difficile and its toxin. Ten specimens (20%) were positive by all three methods. An additional eight specimens were toxin positive only by counterimmunoelectrophoresis. Although counterimmunoelectrophoresis and tissue culture are of equivalent sensitivity, the additional dilution necessary for tissue culture assay may be critical when only small concentrations of toxin are present.
摘要
采用细菌分离、对流免疫电泳和组织培养三种方法对50份粪便标本进行艰难梭菌及其毒素检测。有10份标本（20%）三种方法检测均呈阳性。另外有8份标本仅对流免疫电泳检测毒素呈阳性。虽然对流免疫电泳和组织培养敏感性相当，但当仅存在低浓度毒素时，组织培养检测所需的额外稀释可能至关重要。
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