通过直接气相色谱法检测粪便中的艰难梭菌。
Detection of Clostridium difficile in faeces by direct gas liquid chromatography.
作者信息
Levett P N
出版信息
J Clin Pathol. 1984 Feb;37(2):117-9. doi: 10.1136/jcp.37.2.117.
Abstract
Stool specimens examined for the presence of Clostridium difficile and its cytotoxin were screened by gas liquid chromatography for the presence of volatile fatty acids and p-cresol. Twenty seven of 110 (25%) stools yielded C difficile or cytotoxin; iso-valeric acid was detected in 63/110 (57%) and iso-caproic acid in 18/110 (16%) stools. Para-cresol was found in 24/71 (34%) stools examined. Iso-valeric acid was detected in 85% of stools positive for C difficile, whereas iso-caproic acid (41%) and p-cresol (52%) were found in much lower numbers of C difficile-positive stools. It is concluded that gas chromatographic detection of volatile fatty acids or p-cresol in faeces are not satisfactory screening tests for the presence of C difficile.
摘要
对粪便标本进行艰难梭菌及其细胞毒素检测时，通过气相色谱法筛查挥发性脂肪酸和对甲酚的存在情况。110份粪便标本中有27份（25%）检出艰难梭菌或细胞毒素；63/110（57%）的粪便中检测到异戊酸，18/110（16%）的粪便中检测到异己酸。在所检测的71份粪便中有24份（34%）发现了对甲酚。在85%的艰难梭菌阳性粪便中检测到异戊酸，而在艰难梭菌阳性粪便中，异己酸（41%）和对甲酚（52%）的检出数量要少得多。得出的结论是，粪便中挥发性脂肪酸或对甲酚的气相色谱检测对于艰难梭菌的存在并非令人满意的筛查试验。
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