艰难梭菌的气相色谱鉴定及改良选择性培养基中产细胞毒素的检测
Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium.
作者信息
Levett P N, Phillips K D
出版信息
J Clin Pathol. 1985 Jan;38(1):82-5. doi: 10.1136/jcp.38.1.82.
Abstract
A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.
摘要
本文描述了一种对现有艰难梭菌选择性培养基的改良方法。在培养基中加入DL-正亮氨酸和对羟基苯乙酸，通过气相色谱法检测己酸和对甲酚，能够直接从初次分离平板上鉴定出艰难梭菌。从选择性培养基中取出的琼脂块也可用于体外检测细胞毒素的产生。
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本文引用的文献
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Rapid identification of Clostridium difficile by toxin detection.通过毒素检测快速鉴定艰难梭菌。
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Rapid detection and presumptive identification of Clostridium difficile by p-cresol production on a selective medium.通过在选择性培养基上检测对甲酚来快速检测和初步鉴定艰难梭菌。
J Clin Pathol. 1981 Jun;34(6):642-4. doi: 10.1136/jcp.34.6.642.
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Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species.艰难梭菌及相关菌种的乙二胺四乙酸可溶性抗原的免疫学分析。
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