Verma Neelam, Trehan Nitu, Singh Minni
Biosensor Development Group, Department of Biotechnology & Food Technology, Punjabi University Patiala, 147002, Punjab, India.
Department of Biotechnology, Mata Gujri College Fatehgarh Sahib, 140407, Punjab, India.
Anal Biochem. 2025 Jul;702:115846. doi: 10.1016/j.ab.2025.115846. Epub 2025 Mar 14.
Chlorogenic acid (CGA), a key phenolic acid found in coffee, fruits, vegetables, and herbs, has significant pharmacological activities, necessitating its accurate detection in complex matrices. In this study, an organic acrylate molecularly imprinted polymers-chitosan modified zinc sulphide quantum dots/polydopamine (MIPs-CS:ZnS QDs/PDA) based composite sensor for the detection of CGA has been designed. In MIPs shell, CGA served as template and 4-vinylpyridine and methacrylic acid as functional monomers, azobisisobutyronitrile acting as the initiator and ethylene glycol dimethacrylate as the cross-linker. Chitosan was incorporated to enhance the stability of ZnS QDs, while polydopamine was introduced during polymerization to improve adhesion and the selectivity of MIPs for CGA. Under ideal conditions, the composite sensor had shown a linear range of 0.02-11 μg/mL with detection limit of 8.9 × 10 μg/mL. The composite sensor showed imprinting factor of 6.3, and response time of 12 min. The sensor demonstrated good selectivity towards CGA, in the presence of interfering agents. Composite sensor was successfully applied to detect CGA in plant extracts, coffee and fruit juices, with recovery ranges from 88.93 to 98.49 %. The MIPs-CS:ZnS QDs/PDA composite sensor offers a simple and robust approach for CGA detection in real samples without requiring pre-treatment.
绿原酸（CGA）是一种存在于咖啡、水果、蔬菜和草药中的关键酚酸，具有显著的药理活性，因此需要在复杂基质中对其进行准确检测。在本研究中，设计了一种基于有机丙烯酸酯分子印迹聚合物-壳聚糖修饰硫化锌量子点/聚多巴胺（MIPs-CS:ZnS QDs/PDA）的复合传感器用于检测CGA。在MIPs壳层中，CGA作为模板，4-乙烯基吡啶和甲基丙烯酸作为功能单体，偶氮二异丁腈作为引发剂，乙二醇二甲基丙烯酸酯作为交联剂。引入壳聚糖以增强ZnS QDs的稳定性，而在聚合过程中引入聚多巴胺以提高附着力和MIPs对CGA的选择性。在理想条件下，复合传感器的线性范围为0.02-11μg/mL，检测限为8.9×10μg/mL。复合传感器的印迹因子为6.3，响应时间为12分钟。在存在干扰剂的情况下，该传感器对CGA表现出良好的选择性。复合传感器已成功应用于检测植物提取物、咖啡和果汁中的CGA，回收率范围为88.93%至98.49%。MIPs-CS:ZnS QDs/PDA复合传感器为实际样品中CGA的检测提供了一种简单而可靠的方法，无需预处理。
