Metz A L, Walser M M, Olson W G
J Nutr. 1985 Jul;115(7):929-35. doi: 10.1093/jn/115.7.929.
Large white male turkey poults were fed diets with different levels of vitamins A and D to study the interaction of these vitamins with regard to skeletal development. Poults fed a basal diet deficient in both vitamins A and D developed severe lameness, growth depression, mortality and lesions consistent with rickets. Birds fed a diet containing the required level of vitamin D (900 ICU/kg, NRC estimated requirement) and a high level of vitamin A (400,000 IU/kg) also developed severe lameness, growth depression and a rachiticlike condition, characterized by thicker than normal proximal tibial epiphyseal plates and lower than normal bone mineral content. When fed a diet containing the required level of vitamin A (4,000 IU/kg, NRC estimated requirement) and a high level of vitamin D (900,000 ICU/kg), poults developed hypervitaminosis D as evidenced by mild growth depression and renal tubular mineralization. When poults were fed a diet containing high levels of both vitamins A and D growth rate and bone mineral content were similar to control poults fed a diet containing the required levels of vitamins A and D. In addition, lameness and renal tubular mineralization were not apparent in the poults fed a diet containing high levels of both vitamins A and D. It was concluded that there is an antagonistic interaction between vitamins A and D.
用含有不同水平维生素A和维生素D的日粮饲喂大型白色雄性火鸡幼雏，以研究这些维生素在骨骼发育方面的相互作用。饲喂缺乏维生素A和维生素D的基础日粮的幼雏出现严重跛行、生长抑制、死亡以及与佝偻病一致的病变。饲喂含有所需水平维生素D（900国际雏鸡单位/千克，美国国家研究委员会估计需求量）和高水平维生素A（400,000国际单位/千克）日粮的禽类也出现严重跛行、生长抑制和类似佝偻病的状况，其特征为胫骨近端骨骺板比正常厚且骨矿物质含量低于正常水平。当饲喂含有所需水平维生素A（4,000国际单位/千克，美国国家研究委员会估计需求量）和高水平维生素D（900,000国际雏鸡单位/千克）的日粮时，幼雏出现维生素D过多症，表现为轻度生长抑制和肾小管矿化。当给幼雏饲喂含有高水平维生素A和维生素D的日粮时，其生长速度和骨矿物质含量与饲喂含有所需水平维生素A和维生素D日粮的对照幼雏相似。此外，在饲喂含有高水平维生素A和维生素D日粮的幼雏中，跛行和肾小管矿化并不明显。得出的结论是，维生素A和维生素D之间存在拮抗作用。
