Wang Chong, Wang Huixin, Ni Qingting, Zhou Wenjuan, Liu Dan
School of Chemistry and Chemical Engineering, Jiangsu University of Technology, Changzhou, 213001, PR China.
Department of Geriatric, Wuxi People'S Hospital Affiliated to Nanjing Medical University, Wuxi, 214023, PR China.
Mikrochim Acta. 2025 Mar 17;192(4):236. doi: 10.1007/s00604-025-07107-y.
A water-stable ratiometric fluorescence probe based on casein-Tb-TiC quantum dots was developed for the highly sensitive and selective detection of ciprofloxacin. With the increase in ciprofoxacin concentration, the fluorescence intensity at 440 nm remained relatively stable, while the fluorescence emission at 543 nm exhibited progressive enhancement. This may be attributed to ciprofloxacin being able to effectively coordinate with Tb ions while displacing surrounding water molecules, leading to enhanced luminescence. Thus, the I/I ratio was utilized as an indicator for monitoring changes in ciprofloxacin concentration. The ratiometric fluorescence probe demonstrated excellent linearity across a concentration range 0.1-800 µM and exhibited high sensitivity, achieving a detection limit of 0.018 μM for ciprofloxacin. Furthermore, the ratiometric fluorescence probe was successfully employed for the detection of ciprofloxacin in pork, beef, fish, honey, eggs, and milk samples, achieving recoveries that ranged from 76.5 to 123%. Owing to its advantages of high sensitivity and excellent selectivity, the ratiometric fluorescence probe demonstrates significant potential for practical applications in ciprofloxacin detection.
基于酪蛋白-Tb-TiC量子点开发了一种水稳定的比率荧光探针，用于高灵敏和选择性地检测环丙沙星。随着环丙沙星浓度的增加，440nm处的荧光强度保持相对稳定，而543nm处的荧光发射则逐渐增强。这可能归因于环丙沙星能够有效地与Tb离子配位，同时取代周围的水分子，从而导致发光增强。因此，I/I比率被用作监测环丙沙星浓度变化的指标。该比率荧光探针在0.1-800μM的浓度范围内表现出优异的线性，具有高灵敏度，对环丙沙星的检测限为0.018μM。此外，该比率荧光探针成功用于猪肉、牛肉、鱼肉、蜂蜜、鸡蛋和牛奶样品中环丙沙星的检测，回收率在76.5%至123%之间。由于其高灵敏度和优异选择性的优点，该比率荧光探针在环丙沙星检测的实际应用中显示出巨大潜力。
