Li Shuang, Wang Haijun, Wang Jinghan, Xu Feng, Li Hongmei
Qiqihar Medical University, Qiqihar, China.
PLoS One. 2024 Dec 19;19(12):e0312156. doi: 10.1371/journal.pone.0312156. eCollection 2024.
Ciprofloxacin is a widely used antibiotic in medicine and agriculture. It can cause pollution to the environment and food, thereby affecting human health.
This study proposes the preparation of molecular imprinted fluorescent sensors and their selective detection of ciprofloxacin, with the aim of achieving specific recognition and accurate detection of ciprofloxacin.
Rare earth metal Er3+ is added to ZnS quantum dots to prepare a molecularly imprinted fluorescence sensor (MIP/Er3+/ZnS QDs). The effects of substance concentration, pH value, and time on the fluorescence detection intensity are analyzed to determine the optimal fluorescence detection conditions.
Experimental results showed that the sensor accurately detected ciprofloxacin with a detection limit of 31 nmol/L and the linear range of 0.1-10 μmol/L. The sensor had a recovery rate of 99.7% to 103.5% for ciprofloxacin in milk samples, with a relative standard deviation of less than 5%, indicating accurate determination of ciprofloxacin content.
Molecularly imprinted fluorescent sensors have enormous application potential in the monitoring and control of ciprofloxacin, and are of great significance for ensuring environmental and food safety.
环丙沙星是医药和农业中广泛使用的抗生素。它会对环境和食品造成污染，进而影响人类健康。
本研究提出制备分子印迹荧光传感器并对环丙沙星进行选择性检测，旨在实现对环丙沙星的特异性识别和准确检测。
将稀土金属铒（Er3+）添加到硫化锌（ZnS）量子点中制备分子印迹荧光传感器（MIP/Er3+/ZnS QDs）。分析物质浓度、pH值和时间对荧光检测强度的影响，以确定最佳荧光检测条件。
实验结果表明，该传感器能准确检测环丙沙星，检测限为31 nmol/L，线性范围为0.1 - 10 μmol/L。该传感器对牛奶样品中环丙沙星的回收率为99.7%至103.5%，相对标准偏差小于5%，表明能准确测定环丙沙星含量。
分子印迹荧光传感器在环丙沙星的监测与控制方面具有巨大的应用潜力，对保障环境和食品安全具有重要意义。
