Shi Qingyang, Wang Yuguang, Zhang Lihong, An Haiyan
Department of Otorhinolaryngology, Head and Neck Surgery, Peking University People's Hospital, Xizhimen South Street, Xicheng District, Beijing, China.
Department of Anesthesiology, Peking University People's Hospital, Xizhimen South Street, Xicheng District, Beijing, China.
Acta Otorhinolaryngol Ital. 2025 Feb;45(1):21-27. doi: 10.14639/0392-100X-N2734.
To assess the suitability of Bama pigs as a model for laryngopharyngeal reflux disease (LPRD) research.
Sixteen 8-month-old male Bama pigs underwent esophageal manometry to determine the precise anatomical positioning of the upper (UES) and lower esophageal sphincters (LES) relative to the incisor teeth, as well as their respective contraction intensities. The pigs were randomly allocated into three experimental groups (n = 6, 5, 5), with each group subjected to Dx PH-probe monitoring. In Group 1, animals were fasted for 24 hours and water-deprived for 6 hours before undergoing pH monitoring under anaesthesia. Group 2 was anaesthetised two hours post-normal feeding and subsequently monitored. Group 3 also received anaesthesia two hours after eating but were monitored in an awake state.
The mean distance from the UES to the incisor teeth was found to be 19.8 ± 1 cm, while the LES was located at 40 ± 2.5 cm. The resting pressure measurements revealed a mean value of 64 ± 12 mmHg for the UES and 20 ± 4 mmHg for the LES in Bama pigs. Laryngopharyngeal pH values across the three groups were 7 ± 0.6, 7 ± 0.5, and 7.4 ± 1.2, respectively, showing no significant differences or reflux events. Similarly, there was no statistically significant difference in the lower oesophageal pH between Group 1 and Group 2.
The Bama pig emerges as a suitable animal model for studying LPRD, given its comparable physiological parameters. The feasibility of establishing a reflux model in Bama pigs and using it to investigate the underlying mechanisms of LPRD is convincingly supported by these findings.
评估巴马猪作为喉咽反流病（LPRD）研究模型的适用性。
对16只8月龄雄性巴马猪进行食管测压，以确定食管上括约肌（UES）和食管下括约肌（LES）相对于切牙的精确解剖位置及其各自的收缩强度。将猪随机分为三个实验组（n = 6、5、5），每组进行Dx pH探头监测。在第1组中，动物在麻醉下进行pH监测前禁食24小时并禁水6小时。第2组在正常喂食后2小时麻醉，随后进行监测。第3组也在进食后2小时接受麻醉，但在清醒状态下进行监测。
发现UES到切牙的平均距离为19.8±1 cm，而LES位于40±2.5 cm处。巴马猪UES的静息压力测量平均值为64±12 mmHg，LES为20±4 mmHg。三组的喉咽pH值分别为7±0.6、7±0.5和7.4±1.2，未显示出显著差异或反流事件。同样，第1组和第2组之间食管下段pH值也无统计学显著差异。
鉴于其相似的生理参数，巴马猪成为研究LPRD的合适动物模型。这些发现有力地支持了在巴马猪中建立反流模型并用于研究LPRD潜在机制的可行性。
