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探索脂肪来源干细胞成骨分化中的纳米RNA网络和时间序列基因表达。

Exploring NamiRNA networks and time-series gene expression in osteogenic differentiation of adipose-derived stem cells.

作者信息

Jin Xin, Lu Yi, Fan Zhihong

机构信息

Department of Plastic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

出版信息

Ann Med. 2025 Dec;57(1):2478323. doi: 10.1080/07853890.2025.2478323. Epub 2025 Mar 18.

DOI:10.1080/07853890.2025.2478323
PMID:40100054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11921168/
Abstract

BACKGROUND

Adipose-derived stem cells (ADSCs) are a type of stem cell found in adipose tissue with the capacity to differentiate into multiple lineages, including osteoblasts. The differentiation of ADSCs into osteoblasts underlies osteogenic and pathological cellular basis in osteoporosis, bone damage and repair.

METHODS

Focused on ADSCs osteogenic differentiation, we conducted mRNA, microRNA expression and bioinformatics analysis, including gene differential expression, time series-based trend analysis, functional enrichment, and generates potential nuclear activating miRNAs (NamiRNA) regulatory network. The screened mRNAs in NamiRNA regulatory network were validated with correlation analysis.

RESULTS

The NamiRNA Regulatory Network reveals 4 mRNAs (C12orf61, MIR31HG, NFE2L1, and PCYOX1L) significantly downregulated in differentiated group and may be associated with ADSCs stemness. Furthermore, the significantly upregulated 10 genes (ACTA2, TAGLN, LY6E, IFITM3, NGFRAP1, TCEAL4, ATP5C1, CAV1, RPSA, and KDELR3) were significantly enriched in osteogenic-related pathways, and negatively correlated with ADSCs cell stemness .

CONCLUSION

These findings uncover potential genes related to ADSCs osteogenic differentiation, and provide theoretical basis for underlying ADSCs osteogenic differentiation and related diseases.

摘要

背景

脂肪来源干细胞（ADSCs）是一种存在于脂肪组织中的干细胞，具有分化为多种细胞谱系的能力，包括成骨细胞。ADSCs向成骨细胞的分化是骨质疏松症、骨损伤和修复中骨生成及病理细胞基础的根本所在。

方法

聚焦于ADSCs的成骨分化，我们进行了mRNA、微小RNA表达及生物信息学分析，包括基因差异表达、基于时间序列的趋势分析、功能富集，并构建了潜在的核激活微小RNA（NamiRNA）调控网络。通过相关性分析对NamiRNA调控网络中筛选出的mRNA进行验证。

结果

NamiRNA调控网络显示，4种mRNA（C12orf61、MIR31HG、NFE2L1和PCYOX1L）在分化组中显著下调，可能与ADSCs的干性有关。此外，10个显著上调的基因（ACTA2、TAGLN、LY6E、IFITM3、NGFRAP1、TCEAL4、ATP5C1、CAV1、RPSA和KDELR3）在成骨相关途径中显著富集，且与ADSCs细胞干性呈负相关。

结论

这些发现揭示了与ADSCs成骨分化相关的潜在基因，为ADSCs成骨分化及相关疾病提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/d4eed2558c42/IANN_A_2478323_F0008_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/056c71a2a76c/IANN_A_2478323_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/c9236dc532dd/IANN_A_2478323_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/8f9ae5bdd8a5/IANN_A_2478323_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/9568b8ac4a46/IANN_A_2478323_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/3b0ec4e1d77a/IANN_A_2478323_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/3350b695b360/IANN_A_2478323_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/83725ee947a0/IANN_A_2478323_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/d4eed2558c42/IANN_A_2478323_F0008_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/056c71a2a76c/IANN_A_2478323_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/c9236dc532dd/IANN_A_2478323_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/8f9ae5bdd8a5/IANN_A_2478323_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/9568b8ac4a46/IANN_A_2478323_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/3b0ec4e1d77a/IANN_A_2478323_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/3350b695b360/IANN_A_2478323_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/83725ee947a0/IANN_A_2478323_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e8a/11921168/d4eed2558c42/IANN_A_2478323_F0008_B.jpg

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