Li Jing, Jin Xiaojuan, Liu Sunan, Lin Tongze, Zhang Cheng, Chen Qiutong, Ma Yunsu
School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, 22004, China.
Xuzhou Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Xuzhou, Jiangsu, 221000, China.
J Fluoresc. 2025 Mar 18. doi: 10.1007/s10895-025-04228-8.
Glucose is a critical energy source for human cells, and maintaining stable glucose levels is essential for normal physiological functions. Abnormal glucose levels can lead to health issues such as diabetes, obesity, and hypoglycemia, highlighting the need for precise and rapid glucose detection methods for clinical diagnostics and health management. In this study, a novel ratiometric fluorescent probe was constructed for glucose detection. Using glucose oxidase (GOx) and horseradish peroxidase (HRP) as enzymatic mediators, the probe employs orange-emitting carbon dots (oCDs) as the detection signal and red-emitting quantum dots (rQDs) as the reference signal. Glucose is oxidized by GOx to produce hydrogen peroxide (HO), which subsequently generates hydroxyl radicals (·OH) under HRP catalysis. The ·OH interacts electrostatically with oCDs, forming non-fluorescent complexes and quenching the oCDs fluorescence. Glucose concentration is quantified by monitoring the fluorescence intensity ratio (I/I) between oCDs and rQDs. The probe has a limit of detection (LOD) of 0.47 µM and a limit of quantification (LOQ) of 1.58 µM. After methodological validation, it has been successfully applied to the detection of glucose in human serum. This study provides a solid basis for accurate glucose monitoring and holds potential for the early diagnosis and treatment of metabolic diseases such as diabetes.
葡萄糖是人体细胞的关键能量来源，维持稳定的血糖水平对正常生理功能至关重要。血糖水平异常会导致糖尿病、肥胖症和低血糖等健康问题，这凸显了临床诊断和健康管理中对精确快速血糖检测方法的需求。在本研究中，构建了一种用于葡萄糖检测的新型比率荧光探针。该探针以葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）作为酶介导剂，采用发射橙色光的碳点（oCDs）作为检测信号，发射红色光的量子点（rQDs）作为参比信号。葡萄糖在GOx作用下被氧化生成过氧化氢（HO），随后在HRP催化下产生羟基自由基（·OH）。·OH与oCDs发生静电相互作用，形成非荧光复合物并淬灭oCDs的荧光。通过监测oCDs与rQDs之间的荧光强度比（I/I）来定量葡萄糖浓度。该探针的检测限（LOD）为0.47 µM，定量限（LOQ）为1.58 µM。经过方法学验证后，已成功应用于人体血清中葡萄糖的检测。本研究为准确监测血糖提供了坚实基础，并在糖尿病等代谢疾病的早期诊断和治疗方面具有潜力。
