College of Chemistry and Environmental Engineering, Shanxi Datong University, Datong 037009, China.
Sensors (Basel). 2019 Feb 7;19(3):674. doi: 10.3390/s19030674.
A novel colorimetric and ratiometric fluorescence sensor was constructed by using carbon quantum dots (CQDs) and o-diaminobenzene (ODB). Unlike ODB by itself, ODB oxide (oxODB) not only emits fluorescence, but also produces ultraviolet (UV) absorption. Therefore, on the basis of the potential optical properties of ODB, glucose oxidase (Gox) and horseradish peroxidase (HRP) were introduced into a CQDs⁻ODB system for the quantitative oxidation of ODB. When glucose is present, it is oxidized by oxygen under the catalytic action of its oxidase to form hydrogen peroxide. Hydrogen peroxide is a strong oxidant that can rapidly oxidize ODB through the catalysis of horseradish peroxidase. oxODB can cause changes in the fluorescence ratio (I/I) and absorbance ratio (A/A₀). At the same time, the color of the detection solution can also change under sunlight and ultraviolet lamps. Therefore, glucose can be quantitatively detected by ratiometric fluorescence and colorimetry simultaneously, and semi-quantitatively detected by observing the colors with sunlight and ultraviolet lamps of 365 nm. This increases not only the convenience but also the accuracy of detection. In addition, this sensor has good selectivity and can be used for the determination of glucose in serum, providing a new idea for the development of blood glucose sensors.
一种新型的比色和比率荧光传感器是通过使用碳量子点 (CQDs) 和邻二氨基苯 (ODB) 构建的。与单独的 ODB 不同，邻二氨基苯氧化物 (oxODB) 不仅会发出荧光，还会产生紫外线 (UV) 吸收。因此，基于 ODB 的潜在光学特性，葡萄糖氧化酶 (Gox) 和辣根过氧化物酶 (HRP) 被引入 CQDs-ODB 体系中，用于 ODB 的定量氧化。当存在葡萄糖时，它在其氧化酶的催化作用下被氧气氧化，形成过氧化氢。过氧化氢是一种强氧化剂，可通过辣根过氧化物酶的催化迅速氧化 ODB。oxODB 会引起荧光比 (I/I) 和吸光度比 (A/A₀) 的变化。同时，在阳光和 365nm 紫外线灯下，检测溶液的颜色也会发生变化。因此，葡萄糖可以通过比率荧光和比色法同时进行定量检测，并通过观察阳光和紫外线灯下的颜色进行半定量检测。这不仅提高了检测的便利性，还提高了检测的准确性。此外，该传感器具有良好的选择性，可用于血清中葡萄糖的测定，为血糖传感器的开发提供了新的思路。
