新种和新种，从……和……的根际土壤中分离得到。

sp. nov. and sp. nov., isolated from the rhizosphere soil of and .

作者信息

Zheng Yaxi, Tang Ting, Mo Ping, Wu Zhilong, Li Kaiqin, Zou Wansheng, Wang Yun, Li Baiyuan

机构信息

Key Laboratory of Agricultural Products Processing and Food Safety in Hunan Higher Education, Hunan University of Arts and Science, Changde 415000, Hunan Province, PR China.

College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, Hunan Province, PR China.

出版信息

Int J Syst Evol Microbiol. 2025 Mar;75(3). doi: 10.1099/ijsem.0.006714.

DOI:10.1099/ijsem.0.006714

PMID:40100705

Abstract

Two novel actinobacteria strains, HUAS MG47 and HUAS YS2, were isolated from the rhizosphere soil of and collected from Changde City, Hunan Province, China. These two strains could form rectiflexible spore chains consisting of short cylindrical spores with smooth surfaces, suggesting that they had typical characteristics of the genus . The cell wall peptidoglycan contained ll-diaminopimelic acid as a diagnostic amino acid. The predominant menaquinones were MK-9 (H), MK-9 (H) and MK-9 (H). The genome sizes of HUAS MG47 and HUAS YS2 were 7.35 and 7.88 Mbp with a G+C content of 72.3 and 72.0 mol%, respectively. Phylogenetic analysis based on the 16S rRNA gene and five housekeeping gene sequences (, , , and ) indicated that strains HUAS MG47 and HUAS YS2 formed an evolutionary lineage with CGMCC 4.7393. However, the values of multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization between these two strains and their closely related relatives indicated that they were clearly different from other known species. Consequently, based on the polyphasic taxonomic study, HUAS MG47 and HUAS YS2 represent two novel species for which the names sp. nov. (HUAS MG47=MCCC 1K09365=JCM 37293) and sp. nov. (HUAS YS2=MCCC 1K08940=JCM 36737) are proposed, respectively.

摘要

从中国湖南省常德市采集的[植物名称1]和[植物名称2]根际土壤中分离出两株新型放线菌菌株，HUAS MG47和HUAS YS2。这两株菌株可形成由表面光滑的短圆柱形孢子组成的直向弯曲的孢子链，表明它们具有该属的典型特征。细胞壁肽聚糖含有ll-二氨基庚二酸作为诊断性氨基酸。主要的甲基萘醌为MK-9(H)、MK-9(H)和MK-9(H)。HUAS MG47和HUAS YS2的基因组大小分别为7.35和7.88 Mbp，G+C含量分别为72.3%和72.0 mol%。基于16S rRNA基因和五个管家基因序列（[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5]）的系统发育分析表明，菌株HUAS MG47和HUAS YS2与[属名]CGMCC 4.7393形成一个进化谱系。然而，这两株菌株与其近缘亲属之间的多位点序列分析进化距离、平均核苷酸同一性和DNA-DNA杂交值表明，它们与其他已知的[属名]物种明显不同。因此，基于多相分类研究，HUAS MG47和HUAS YS2代表两个新的[属名]物种，分别提议命名为[新物种名1] sp. nov.（HUAS MG47 = MCCC 1K09365 = JCM 37293）和[新物种名2] sp. nov.（HUAS YS2 = MCCC 1K08940 = JCM 36737）。