sp. nov., isolated from the rhizosphere soil of .

A novel actinobacterium, strain HUAS 3, was isolated from the rhizosphere soil of collected in Hunan Province, PR China. Strain HUAS 3 contained -diaminopimelic acid in the cell-wall peptidoglycan. The dominant menaquinones were MK-9(H), MK-9(H), MK-10(H) and MK-9(H). The polar lipids consisted of diphosphatidylglycerol, phospholipids, phosphatidylethanolamine, phosphatidylglycerol, phosphotidylinositol and phosphatidylinositol mannosides. The main cellular fatty acids (>5.0 %) were C ω8, iso-C, C ω9, iso-C, C and summed feature 3 (C ω6 and/or C ω7). The DNA G+C content of the novel strain's genome sequence, consisting of 7 196 442 bp, was 72.8 mol%. The full-length 16S rRNA gene sequence analysis indicated that strain HUAS 3 belonged to the genus and showed highest similarities to A38 (99.44 %), DSM 43816 (99.23 %), DSM 45142 (99.23 %), PPF5-17 (99.16 %) and DSM 44398 (98.96 %). Phylogenetic trees based on 16S rRNA gene sequences showed that strain HUAS 3 was closely related to A38, DSM 45142 and PPF5-17. The phylogenomic tree revealed that strain HUAS 3 was closely related to DSM 43817. However, the average nucleotide identity (ANIb/ANIm) and the digital DNA-DNA hybridization values between them were 84.75 /88.16 and 30.80 %, respectively, far less than the 95-96 and 70 % cut-off points recommended for delineating species. Furthermore, strain HUAS 3 was distinct from the type strain of in terms of phenotypic and chemotaxonomic characteristics. In summary, strain HUAS 3 represents a novel species, for which the name sp. nov. is proposed. The type strain is HUAS 3 (=MCCC 1K08599=JCM 36275).