Wang Qianqian, Li Bole, Qiu Zefeng, Ying Jiayi, Jin Xuyichen, Lu Zeyun, Zhang Junli, Chen Xia, Zhu Xiangtao
College of Jiyang, Zhejiang A&F University, Zhuji, 311800, China.
Weifang Vocational College, Weifang, 262737, China.
BMC Genomics. 2025 Mar 18;26(1):266. doi: 10.1186/s12864-025-11439-7.
The complete dormancy release determines the quality of bud break, flowering and fruiting. While in tree peony (Paeonia suffruticosa Andr.), the insufficient accumulation of cold temperatures results in incomplete dormancy release and poor flowering quality.
In order to investigate if phytohormone can replace the chilling requirement in south China and other similar regions, the roles of fluridone (Flu), gibberellin (GA), and their combination in the bud dormancy release process were analyzed. It demonstrated that the application of exogenous GA and the mixture significantly expedited the dormancy release of tree peony at 23℃. Furthermore, the endogenous hormone levels provided evidence for the substantial impact of exogenous GA on dormancy release, highlighting its potential involvement in the chilling-independent pathway of dormancy release. Transcriptome sequencing and analysis of expression profiles were conducted to identify the crucial genes implicated in the dormancy release mechanism of tree peony. Among numerous genes from diverse gene families, the particularly interesting were TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS-like genes (PsTCP3, PsTCP4, and PsTCP14), which had significant expression levels during the dormancy release process under different treatments. They were divided into two distinct sub-families based on their different domains. Specifically, PsTCP14 was classified under Class I, while PsTCP3 and PsTCP4 were classified under Class II. The analysis of expression patterns revealed a significant accumulation of the three PsTCPs in buds undergoing dormancy release, with clear upregulation observed in response to GA and the mixture treatments. Additionally, the analysis of promoter activity demonstrated the sensitivity of PsTCP4 and PsTCP14 to GA and Flu.
The application of exogenous GA has been shown to effectively expedite the release of dormancy in tree peony through a pathway that is not dependent on chilling. Further research found that PsTCP genes might play a crucial role in this process. These findings contribute to the understanding of the molecular mechanism of PsTCPs in the hormone-mediated and temperature-independent release of bud dormancy in tree peony.
完全解除休眠决定了芽萌发、开花和结果的质量。而在牡丹（Paeonia suffruticosa Andr.）中，低温积累不足会导致休眠解除不完全，开花质量差。
为了研究植物激素是否可以替代中国南方及其他类似地区的低温需求，分析了氟啶酮（Flu）、赤霉素（GA）及其组合在芽休眠解除过程中的作用。结果表明，外源GA及其混合物的施用显著加速了23℃下牡丹的休眠解除。此外，内源激素水平为外源GA对休眠解除的重大影响提供了证据，突出了其在不依赖低温的休眠解除途径中的潜在参与。进行了转录组测序和表达谱分析，以鉴定与牡丹休眠解除机制相关的关键基因。在来自不同基因家族的众多基因中，特别有趣的是玉米分枝1、环化酶和增殖细胞因子样基因（PsTCP3、PsTCP4和PsTCP14），它们在不同处理下的休眠解除过程中具有显著的表达水平。根据它们不同的结构域，它们被分为两个不同的亚家族。具体而言，PsTCP14被归类为I类，而PsTCP3和PsTCP4被归类为II类。表达模式分析显示，这三个PsTCPs在经历休眠解除的芽中显著积累，在GA和混合物处理下观察到明显上调。此外，启动子活性分析表明PsTCP4和PsTCP14对GA和Flu敏感。
已表明外源GA的施用可通过不依赖低温的途径有效加速牡丹休眠的解除。进一步研究发现，PsTCP基因可能在此过程中起关键作用。这些发现有助于理解PsTCPs在激素介导的、不依赖温度的牡丹芽休眠解除中的分子机制。
