Wang Qianqian, Li Bole, Qiu Zefeng, Lu Zeyun, Hang Ziying, Wu Fan, Chen Xia, Zhu Xiangtao
College of Jiyang, Zhejiang A&F University, Zhuji 311800, China.
Plants (Basel). 2024 Feb 2;13(3):437. doi: 10.3390/plants13030437.
Tree peony ( Andr.) is a traditional Chinese flower with significant ornamental and medicinal value. Its growth and development process is regulated by some internal and external factors, and the related regulatory mechanism is largely unknown. Myelocytomatosis transcription factors () play significant roles in various processes such as plant growth and development, the phytohormone response, and the stress response. As the identification and understanding of the MYC family in tree peony remains limited, this study aimed to address this gap by identifying a total of 15 in tree peony and categorizing them into six subgroups based on bioinformatics methods. Furthermore, the gene structure, conservative domains, -elements, and expression patterns of the were thoroughly analyzed to provide a comprehensive overview of their characteristics. An analysis in terms of gene structure and conserved motif composition suggested that each subtribe had similarities in function. An analysis of the promoter sequence revealed the presence of numerous -elements associated with plant growth and development, the hormone response, and the stress response. qRT-PCR results and the protein interaction network further demonstrated the potential functions of in the growth and development process. While in comparison to the control, only exhibited a statistically significant variation in expression levels in response to exogenous hormone treatments and abiotic stress. A promoter activity analysis of revealed its sensitivity to Flu and high temperatures, but exhibited no discernible difference under exogenous GA treatment. These findings help establish a basis for comprehending the molecular mechanism by which regulate the growth and development of tree peony.
牡丹（芍药属牡丹组植物）是一种具有重要观赏和药用价值的中国传统花卉。其生长发育过程受一些内部和外部因素调控，相关调控机制大多未知。髓细胞瘤转录因子（MYC）在植物生长发育、植物激素应答和胁迫应答等各种过程中发挥重要作用。由于对牡丹中MYC家族的鉴定和了解仍然有限，本研究旨在通过鉴定牡丹中总共15个MYC并基于生物信息学方法将它们分为六个亚组来填补这一空白。此外，对这些MYC的基因结构、保守结构域、顺式作用元件和表达模式进行了深入分析，以全面概述它们的特征。基因结构和保守基序组成分析表明，每个亚族在功能上具有相似性。启动子序列分析揭示了存在许多与植物生长发育、激素应答和胁迫应答相关的顺式作用元件。qRT-PCR结果和蛋白质相互作用网络进一步证明了MYC在生长发育过程中的潜在功能。与对照相比，在外源激素处理和非生物胁迫下，只有一个MYC在表达水平上表现出统计学上的显著差异。对一个MYC的启动子活性分析表明其对氟和高温敏感，但在外源赤霉素处理下没有明显差异。这些发现有助于为理解MYC调控牡丹生长发育的分子机制奠定基础。
