Deluca Anthony, Bascom Brooke, Key Planas Daniela A, Kocher Matthew A, Torres Marielise, Arbeitman Michelle N
Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL 32306, USA.
iScience. 2025 Feb 15;28(3):112037. doi: 10.1016/j.isci.2025.112037. eCollection 2025 Mar 21.
Animals need to integrate information across neuronal networks that direct reproductive behaviors and circadian rhythms. The Drosophila master regulatory transcription factors that direct courtship and circadian rhythms are co-expressed. We find sex differences in the number of these () and ()-expressing neurons ( ∩ neurons) regulated by male-specific Fru. We assign the ∩ neurons to the electron microscopy connectome and to subtypes of clock neurons. We discover sex differences in -expressing neurons that are post-synaptic targets of -expressing neurons. When ∩ neurons are activated or silenced, we observe a male-specific shortening of period length. Activation of ∩ neurons also changes the rate a courtship behavior is performed. We examine male courtship behavior over 24 h and find courtship activities peak at lights-on. These results reveal how neurons that subserve the two processes can impact behavioral outcomes in a sex-specific manner.
动物需要整合跨越指导生殖行为和昼夜节律的神经网络的信息。指导求偶和昼夜节律的果蝇主调节转录因子是共表达的。我们发现，由雄性特异性Fru调控的这些（）和（）表达神经元（∩神经元）的数量存在性别差异。我们将∩神经元分配到电子显微镜连接体和时钟神经元亚型。我们发现，作为表达神经元突触后靶点的表达神经元存在性别差异。当∩神经元被激活或沉默时，我们观察到周期长度出现雄性特异性缩短。∩神经元的激活也会改变求偶行为的执行速度。我们在24小时内观察雄性求偶行为，发现求偶活动在开灯时达到峰值。这些结果揭示了服务于这两个过程的神经元如何以性别特异性方式影响行为结果。
