Spanoghe Jeroen, Van Acker Arne, Carrette Evelien, Vonck Kristl, Boon Paul, Raedt Robrecht
4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, Belgium.
IBRO Neurosci Rep. 2025 Feb 27;18:378-383. doi: 10.1016/j.ibneur.2025.02.016. eCollection 2025 Jun.
Dimethyl sulfoxide (DMSO) is a commonly used solvent in life sciences due to its excellent ability to dissolve compounds with poor water-solubility. Depending on the applied dose, the variety of DMSO's physiological and biological effects may compromise its suitability as a vehicle molecule. Even low concentrations of DMSO are known to affect neuronal excitability . As effects have not been studied extensively, this exploratory study investigated the effects of intracerebroventricular (ICV) administration of different DMSO concentrations on hippocampal electrophysiology in mice. Acute recordings of hippocampal evoked potentials (EPs) and electroencephalography (EEG) were performed before and after ICV injection of a 5 µl DMSO solution, with concentrations ranging from 2.5 % to 100 % DMSO. Solutions containing up to 50 % DMSO had no acute effects on hippocampal electrophysiology. Administration of 75 % and 100 % DMSO was found to alter evoked responses, indicating increased excitability. Our results indicate that DMSO can be used as a vehicle in volumes of 5 µl containing concentrations of up to 50 % without affecting acute hippocampal electrophysiological studies in mice. Higher concentrations should be avoided as these affect neuronal excitability.
二甲基亚砜（DMSO）因其出色的溶解水溶性差的化合物的能力，是生命科学中常用的溶剂。根据所施用的剂量，DMSO的各种生理和生物学效应可能会影响其作为载体分子的适用性。即使是低浓度的DMSO也已知会影响神经元兴奋性。由于尚未对其效应进行广泛研究，本探索性研究调查了脑室内（ICV）注射不同浓度DMSO对小鼠海马体电生理学的影响。在脑室内注射5μl DMSO溶液（浓度范围为2.5%至100% DMSO）之前和之后，对海马体诱发电位（EPs）和脑电图（EEG）进行急性记录。含有高达50% DMSO的溶液对海马体电生理学没有急性影响。发现施用75%和100% DMSO会改变诱发反应，表明兴奋性增加。我们的结果表明，DMSO可以作为载体，以5μl的体积使用，浓度高达50%，而不会影响小鼠的急性海马体电生理研究。应避免使用更高的浓度，因为这些浓度会影响神经元兴奋性。
