Diagnostics and immunological function of CENPN in human tumors: from pan-cancer analysis to validation in breast cancer.

BACKGROUND

Centromere protein N (), a member of the centromere protein family, contributes to ribonucleic assembly, mitosis progression, and chromosome separation. manifests a close link with the occurrence and progression of several malignant cancers, but there is no pan-cancer study on , and we aim to ascertain the connection between and human cancer prognosis and immunotherapy.

METHODS

The function in multiple malignant tumors was comprehensively investigated with data from The Cancer Genome Atlas (TCGA) and integrated Gene Expression Omnibus (GEO) database. We examined the transcriptional level, prognostic effect, diagnostic value, genetic and epigenetic alteration, methylation level, and immunological importance of . Furthermore, this work provided further confirmation of the phenotypic regulating function of in breast cancer (BC) cells.

RESULTS

exhibited significant upregulation in diverse cancer tissues and had different expression patterns across immunological and molecular subgroups in several cancer types. Elevated expression of may correlate with a worse prognosis. effectively differentiates most cancers from healthy tissues. Hypomethylate was shown to be promoter in most cancers. was shown to be connected with levels of different immune cell infiltration. Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Set Enrichment Analysis (GSEA) analysis suggested that may mediate neutrophil extranuclear trap formation, cell cycle, and P53 signaling pathways in cancer. studies showed that the overexpression of promotes the proliferation, invasion, and migration of BC cells, while concurrently inhibiting their apoptosis.

CONCLUSIONS

may operate as a novel predictive indicator and molecular target for targeted therapy in pan-cancer. Significantly, contributed to controlling the BC growth and advancement.