Tao Jia-Yu, Zhu Jun, Gao Yu-Qiong, Jiang Min, Yin Hong
Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Thoracic Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.
Transl Cancer Res. 2025 Feb 28;14(2):1479-1491. doi: 10.21037/tcr-2025-128. Epub 2025 Feb 26.
The conventional in vitro research on tumor mechanisms is typically based on two-dimensional (2D) culture of tumor cells, which has many limitations in replicating tumorigenesis processes. In contrast, the three-dimensional (3D) bioprinting has paved the way for the construction of more biomimetic in vitro tumor models. This article comprehensively elucidates the features of 3D bioprinting and meticulously summarizes its applications in several selected tumors, aiming to offer valuable insights for future relevant studies.
A literature search was conducted in the databases of PubMed and Web of Science for articles on 3D bioprinting for tumor model construction.
This article introduces various 3D bioprinting technologies for tumor model construction, focusing on their pros and cons, principles, and protocols. Several tumor models are presented, detailing their utility in tumorigenesis research and their constraints. To date, 3D bioprinting has been widely applied in oncology, addressing the limitation of traditional 2D tumor cell culture in replicating tumor microenvironment (TME).
Advanced 3D bioprinting technology accurately replicates the complex TME and the heterogeneity of intratumor structures, enabling further tumor studies. It significantly fuels our understanding of tumor pathophysiology and offers new hope for cancer patients.
传统的肿瘤机制体外研究通常基于肿瘤细胞的二维（2D）培养，在复制肿瘤发生过程方面存在诸多局限性。相比之下，三维（3D）生物打印为构建更具仿生学的体外肿瘤模型铺平了道路。本文全面阐述了3D生物打印的特点，并精心总结了其在几种特定肿瘤中的应用，旨在为未来的相关研究提供有价值的见解。
在PubMed和Web of Science数据库中进行文献检索，以获取有关用于肿瘤模型构建的3D生物打印的文章。
本文介绍了用于肿瘤模型构建的各种3D生物打印技术，重点阐述了它们的优缺点、原理和操作流程。展示了几种肿瘤模型，详细说明了它们在肿瘤发生研究中的效用及其局限性。迄今为止，3D生物打印已在肿瘤学中得到广泛应用，克服了传统2D肿瘤细胞培养在复制肿瘤微环境（TME）方面的局限性。
先进的3D生物打印技术能够准确复制复杂的TME和肿瘤内部结构的异质性，有助于进一步开展肿瘤研究。它极大地增进了我们对肿瘤病理生理学的理解，并为癌症患者带来了新的希望。
