Xu Dong, Shan Lei, Guo Bei, Wang Jing, Huang Qiang, Wang Shujun, Li Fuhou, Wu Shaojie, Wang Weixia, Chen Jinri
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou 222005, China; Jiangsu Key Laboratory of Marine Biotechnology, 59 Cangwu Road, Haizhou 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, 59 Cangwu Road, Haizhou 222005, China.
State Key Laboratory of Genetic Engineering, MOE Engineering Research Centre of Gene Technology, School of Life Sciences, Fudan University, Shanghai 200438, China.
Food Chem. 2025 Jun 30;478:143291. doi: 10.1016/j.foodchem.2025.143291. Epub 2025 Feb 8.
High kanamycin (KAN) residue in the human body can seriously threaten human health. Therefore, there is an urgent need to develop an accurate, sensitive and simple biosensor to monitor KAN in food. Herein, an aptasensor for KAN has been developed by employing the split aptamer, DNA-templated silver nanoclusters (DNA-AgNCs), and Exonuclease I (Exo I). The aptasensor is composed of a signal recognition and transduction system consisting of split aptamers Apt1, Apt2 and the complementary sequence cDNA of Apt1, and an Exo I-assisted target recovery signal amplification system. The fluorescence signal of the aptasensor can be amplified by 48.98 % by Exo I. And the aptasensor can achieve an ultra-low detection limit of 1.07 nM with a good linear range of 5 nM to 50 nM and 75 nM to 1 μM. Meanwhile, the aptasensor has the characteristics of simple preparation, and convenient use, which has obvious advantages to detect KAN.
人体内高浓度的卡那霉素（KAN）残留会严重威胁人体健康。因此，迫切需要开发一种准确、灵敏且简便的生物传感器来监测食品中的KAN。在此，通过采用分裂适体、DNA模板化银纳米簇（DNA-AgNCs）和核酸外切酶I（Exo I）开发了一种用于KAN的适体传感器。该适体传感器由一个信号识别和转导系统组成，该系统由分裂适体Apt1、Apt2和Apt1的互补序列cDNA组成，以及一个Exo I辅助的目标回收信号放大系统。Exo I可使适体传感器的荧光信号放大48.98%。该适体传感器可实现1.07 nM的超低检测限，在5 nM至50 nM和75 nM至1 μM的良好线性范围内。同时，该适体传感器具有制备简单、使用方便的特点在检测KAN方面具有明显优势。
