Ecology and Conservation Laboratory, Federal University of Fronteira Sul, Erechim, RS, 99.700-000, Brazil.
Sci Rep. 2023 Oct 19;13(1):17826. doi: 10.1038/s41598-023-45090-7.
This study evaluated the lethal, sublethal, and toxic of a commercial formulation of cypermethrin in the anuran species Physalaemus gracilis. In the acute test, concentrations of 100-800 μg L were tested over 96 h. In the chronic test, cypermethrin concentrations recorded in nature (1, 3, 6, and 20 μg L) were tested for mortality and then used for the micronucleus test and erythrocyte nuclear abnormalities over a 7-days period. The LC determined for P. gracilis for the commercial cypermethrin formulation was 273.41 μg L. In the chronic test, a mortality of more than 50% was observed at the highest concentration (20 μg L), as it caused half of the tadpoles studied to die. The micronucleus test showed significant results at concentrations of 6 and 20 μg L and recorded the presence of several nuclear abnormalities, indicating the genotoxic potential of the commercial cypermethrin formulation for P. gracilis. Cypermethrin presented a high risk to the species, indicating that it has the potential to cause several problems in the short and long term and to affect the dynamics of this ecosystem. Therefore, it can be concluded that the commercial formulation of cypermethrin had toxicological effects on P. gracilis.
本研究评估了一种拟除虫菊酯商业制剂对 Physalaemus gracilis 无尾两栖物种的致死、亚致死和毒性作用。在急性试验中，测试了浓度为 100-800μg/L 的制剂，时间为 96 小时。在慢性试验中，测试了自然界中记录到的拟除虫菊酯浓度（1、3、6 和 20μg/L）对死亡率的影响，然后在 7 天内进行微核试验和红细胞核异常检测。确定 P. gracilis 对商业拟除虫菊酯制剂的 LC 值为 273.41μg/L。在慢性试验中，在最高浓度（20μg/L）下观察到死亡率超过 50%，因为它导致研究中的一半蝌蚪死亡。微核试验在 6 和 20μg/L 浓度下显示出显著结果，并记录了几种核异常的存在，表明商业拟除虫菊酯制剂对 P. gracilis 具有遗传毒性潜力。拟除虫菊酯对该物种具有高风险，表明它有可能在短期和长期内造成多种问题，并影响该生态系统的动态。因此，可以得出结论，商业拟除虫菊酯制剂对 P. gracilis 具有毒理学影响。
