Department of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Department of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Ultrasound Med Biol. 2021 Jul;47(7):1868-1880. doi: 10.1016/j.ultrasmedbio.2021.02.025. Epub 2021 Apr 6.
Timely angiogenesis and effective microcirculation perfusion are essential for the survival and functional recovery of transplanted ovaries. Ultrasound-targeted microbubble destruction (UTMD) can lead to angiogenesis and increase flow perfusion by causing transient inflammation. The purpose of this study was to evaluate the effects of UTMD on transplanted ovarian revascularization and survival. In vitro, for the criteria of cell viability and tube formation capability, the optimal exposure parameters were determined to be a microbubble concentration of 1 × 10/mL, mechanical index of 1 and exposure time of 30 s. After ovarian transplantation, 40 female Sprague Dawley rats were divided into four groups: transplantation alone, ultrasound alone, microbubbles alone and ultrasound and microbubbles (UTMD). At 7 d after transplantation, ovarian perfusion was assessed using qualitative and quantitative methods. The effect of angiogenesis was assessed by contrast-enhanced ultrasound, laser Doppler perfusion imaging and histologic analysis. The results, in which ovarian perfusion was highest in the UTMD group, suggest that UTMD can effectively improve ovarian perfusion. Compared with the other three groups, the number of follicles, microvascular density and rate of Ki-67-positive cells increased significantly in the UTMD group, while apoptosis decreased significantly (p < 0.05). The study indicates that UTMD promoted ovarian re-vascularization after ovarian transplantation and maintained follicular reserve.
及时的血管生成和有效的微循环灌注对于移植卵巢的存活和功能恢复至关重要。超声靶向微泡破坏（UTMD）可以通过引起短暂的炎症来导致血管生成和增加血流灌注。本研究旨在评估 UTMD 对移植卵巢再血管化和存活的影响。在体外，根据细胞活力和管形成能力的标准，确定了最佳的暴露参数为微泡浓度为 1×10/mL、机械指数为 1 和暴露时间为 30 s。卵巢移植后，将 40 只雌性 Sprague Dawley 大鼠分为四组：单纯移植组、单纯超声组、单纯微泡组和超声联合微泡组（UTMD 组）。移植后 7 d，采用定性和定量方法评估卵巢灌注。通过对比增强超声、激光多普勒灌注成像和组织学分析评估血管生成的效果。结果表明，UTMD 组卵巢灌注最高，提示 UTMD 可有效改善卵巢灌注。与其他三组相比，UTMD 组的卵泡数量、微血管密度和 Ki-67 阳性细胞的比例明显增加，而凋亡明显减少（p<0.05）。研究表明，UTMD 促进了卵巢移植后的再血管化，维持了卵泡储备。
