Figueiredo José Edson Fontes, Diniz Gisele de Fátima Dias, Marins Mikaely Sousa, Silva Felipe Campos, Ribeiro Vitória Palhares, Lanza Fabrício Eustáquio, de Oliveira-Paiva Christiane Abreu, Cruz-Magalhães Valter
Biochemistry Molecular Laboratory, Embrapa Maize and Sorghum Research Center, Sete Lagoas, Brazil.
Soil Microbiology Laboratory, Embrapa Maize and Sorghum Research Center, Sete Lagoas, Brazil.
Front Microbiol. 2025 Mar 5;16:1522136. doi: 10.3389/fmicb.2025.1522136. eCollection 2025.
is a ubiquitous bacterium with potent antifungal activity and a plant growth promoter. This study investigated the potential of CNPMS-22 as a biocontrol agent against phytopathogenic fungi under diverse experimental conditions and its potential as a plant growth promoter. Genome sequencing and analysis revealed putative genes involved in these traits.
This research performed experiments to evaluate the CNPMS-22 antagonistic activity against 10 phytopathogenic fungi using dual culture in plate (DCP) and inverted sealed plate assay (ISP). Greenhouse and field tests evaluated the ability of CNPMS-22 to control in maize plants . The CNPMS-22 genome was sequenced using the Illumina HiSeq 4,000 platform, and genomic analysis also included manual procedures to identify genes of interest accurately.
CNPMS-22 showed antifungal activity against all fungi tested, with notable reductions in mycelial growth in both DCP and ISP experiments. In the ISP, volatile organic compounds (VOCs) produced by CNPMS-22 also altered the mycelium coloration of some fungi. Scanning electron microscopy revealed morphological alterations in the hyphae of . in contact with CNPMS-22, including twisted, wrinkled, and ruptured hyphae. Eight cluster candidates for synthesizing non-ribosomal lipopeptides and ribosomal genes for extracellular lytic enzymes, biofilm, VOCs, and other secondary metabolites with antifungal activity and plant growth promoters were identified by genomic analysis. The greenhouse and field experiments showed that seed treatment with CNPMS-22 reduced symptoms in plants and increased maize productivity.
Our findings highlight the CNPMS-22's potential as bioinoculant for fungal disease control and plant growth with valuable implications for a sustainable crop productivity.
是一种具有强大抗真菌活性的普遍存在的细菌，也是一种植物生长促进剂。本研究调查了CNPMS - 22在不同实验条件下作为植物病原真菌生物防治剂的潜力及其作为植物生长促进剂的潜力。基因组测序和分析揭示了与这些性状相关的假定基因。
本研究进行了实验，使用平板双培养（DCP）和倒置密封平板试验（ISP）评估CNPMS - 22对10种植物病原真菌的拮抗活性。温室和田间试验评估了CNPMS - 22在玉米植株中防治的能力。使用Illumina HiSeq 4000平台对CNPMS - 22基因组进行测序，基因组分析还包括手动程序以准确识别感兴趣的基因。
CNPMS - 22对所有测试真菌均表现出抗真菌活性，在DCP和ISP实验中菌丝体生长均显著减少。在ISP中，CNPMS - 22产生的挥发性有机化合物（VOCs）也改变了一些真菌的菌丝体颜色。扫描电子显微镜显示与CNPMS - 22接触的的菌丝体形态发生改变，包括菌丝扭曲、起皱和破裂。通过基因组分析鉴定了八个用于合成非核糖体脂肽的簇候选基因以及用于细胞外裂解酶、生物膜、VOCs和其他具有抗真菌活性和植物生长促进剂的次生代谢物的核糖体基因。温室和田间试验表明，用CNPMS - 22处理种子可减轻植物症状并提高玉米产量。
我们的研究结果突出了CNPMS - 22作为真菌病害防治生物接种剂和促进植物生长的潜力，对可持续作物生产力具有重要意义。
