Jain Vedant D, Gormly Celeste J, Lundquist Erik A
Molecular Biosciences, University of Kansas, Lawrence, Kansas, United States.
MicroPubl Biol. 2025 Mar 4;2025. doi: 10.17912/micropub.biology.001526. eCollection 2025.
Previous studies showed that the apically secreted cuticular collagens DPY-17 , SQT-3 , and DPY-14 control the left-right asymmetric migration of the Q neuroblasts in . Furthermore, apical secretion of DPY-17 and SQT-3 require the BLI-4 proprotein convertase of the subtilisin/kexin family and the consensus furin cleavage site (CFCS) in the N-terminus of DPY-17 and SQT-3 . Work here shows that the CFCS sites of DPY-17 and SQT-3 are required for their roles in Q neuroblast migration. mutants had only weak effects on Q neuroblast migration, possibly due to redundancy among isoforms. These results suggest that apical secretion of cuticular collagens is required for Q neuroblast migration. These collagens might themselves provide left-right asymmetric guidance information, or might regulate another aspect of Q cell interaction with the cuticle, such as adhesion.
先前的研究表明，顶端分泌的表皮胶原蛋白DPY-17、SQT-3和DPY-14控制着Q神经母细胞在[具体位置未提及]中的左右不对称迁移。此外，DPY-17和SQT-3的顶端分泌需要枯草杆菌蛋白酶/kexin家族的BLI-4前蛋白转化酶以及DPY-17和SQT-3 N端的共有弗林蛋白酶切割位点（CFCS）。本文的研究表明，DPY-17和SQT-3的CFCS位点对于它们在Q神经母细胞迁移中的作用是必需的。[具体突变体未提及]突变体对Q神经母细胞迁移的影响较弱，这可能是由于异构体之间的冗余所致。这些结果表明，表皮胶原蛋白的顶端分泌是Q神经母细胞迁移所必需的。这些胶原蛋白可能自身提供左右不对称的引导信息，或者可能调节Q细胞与表皮相互作用的另一个方面，例如黏附。
