Liu Junxi, Liu Youyun, Wang Feifei, Yuan Yonglei, Ma Hongyu, Qu Liping
Yunnan Botanee Bio-Technology Group Co., Ltd., Kunming, China.
Yunnan Characteristic Plant Extraction Laboratory, Yunnan Characteristic Plant Extraction Laboratory Co., Ltd., Kunming, China.
Front Pharmacol. 2025 Mar 6;16:1520392. doi: 10.3389/fphar.2025.1520392. eCollection 2025.
The root of Andrews ( Andr.), is a traditional Chinese medicine. Numerous studies have shown that it possesses anti-inflammatory, antioxidant, and anti-aging effects due to its rich content of bioactive compounds such as polyphenols and paeonol. Thus, it finds extensively applied in the fields of medicine and cosmetics. However, there are few reports on the photoprotective effects of Andr. root bark, this study aims to investigate its research in this area.
This study utilized Andr. root bark sourced from Kunming, Yunnan Province, China. The Andr. root extract (PSAE) was obtained using AB-8 resin. The photoprotective effect of PSAE was assessed using HaCaT cells, HFF cells, and a 3D Reconstructed Human full T-Skin™ model. Mechanistic investigations were performed using RT-qPCR, WB, IF, H&E staining, Masson's trichrome staining and IHC staining. Finally, an assessment of the effects on humans was conducted.
The total phenolic content in the obtained PSAE was 48.9%. Antioxidant activity studies demonstrated that PSAE effectively inhibits DPPH radicals, superoxide anions, hydroxyl radicals, and ABTS radicals, while also enhancing the inhibition rates of collagenase and hyaluronidase. studies on photoaging resistance revealed that PSAE significantly reduced the UV-induced increases in reactive oxygen species (ROS) levels and senescence-associated β-galactosidase (SA-β-gal) activity. Mechanistic studies indicated that PSAE suppressed the overexpression of IRS1 and its downstream effectors, including PI3K, AKT, and mTOR induced by UV irradiation. A human efficacy assessment was conducted by evaluating parameters such as transepidermal water loss (TEWL), epidermal moisture content, roughness and elasticity, confirming the efficacy of PSAE in humans.
In summary, PSAE attenuates UV-induced oxidative damage, genetic damage, and collagen degradation associated with photoaging by modulating the IRS/PI3K/FOXO signaling pathway. This study elucidated the mechanism through which PSAE, thereby providing strong support for its application in cosmetic anti-aging formulations.
地锦（Andr.）的根是一种传统中药。大量研究表明，由于其富含多酚和丹皮酚等生物活性化合物，它具有抗炎、抗氧化和抗衰老作用。因此，它在医药和化妆品领域得到广泛应用。然而，关于地锦根皮的光保护作用的报道较少，本研究旨在探讨其在该领域的研究。
本研究使用了来自中国云南省昆明市的地锦根皮。地锦根提取物（PSAE）采用AB - 8树脂获得。使用HaCaT细胞、HFF细胞和3D重建人全层皮肤模型评估PSAE的光保护作用。使用RT - qPCR、WB、IF、H&E染色、Masson三色染色和IHC染色进行机制研究。最后，对人体效果进行评估。
所得PSAE中的总酚含量为48.9%。抗氧化活性研究表明，PSAE有效抑制DPPH自由基、超氧阴离子、羟基自由基和ABTS自由基，同时还提高了胶原酶和透明质酸酶的抑制率。抗光老化研究表明，PSAE显著降低紫外线诱导的活性氧（ROS）水平和衰老相关β - 半乳糖苷酶（SA - β - gal）活性的升高。机制研究表明，PSAE抑制紫外线照射诱导的IRS1及其下游效应物（包括PI3K、AKT和mTOR）的过表达。通过评估经表皮水分流失（TEWL）、表皮水分含量、粗糙度和弹性等参数进行人体功效评估，证实了PSAE在人体中的功效。
总之，PSAE通过调节IRS/PI3K/FOXO信号通路减轻紫外线诱导的与光老化相关的氧化损伤、基因损伤和胶原蛋白降解。本研究阐明了PSAE的作用机制，从而为其在化妆品抗老化配方中的应用提供了有力支持。
