Guan Tangfei, Yang Xin, Hong Canhui, Zhang Zehao, Xiao Peiyun, Yang Yongshou, Zhang Chenggui, He Zhengchun
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China.
National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China.
Curr Issues Mol Biol. 2025 Aug 4;47(8):619. doi: 10.3390/cimb47080619.
Alopecia, a prevalent dermatological disorder affecting over half of the global population, is strongly associated with psychological distress. Extracts from (L. PA), a medicinal insect resource, exhibit pharmacological activities (e.g., antioxidant, anti-inflammatory, microcirculation improvement) that align with core therapeutic targets for alopecia. This study aimed to systematically investigate the efficacy and mechanisms of PA extracts in promoting hair regeneration. A strategy combining network pharmacology prediction and in vivo experiments was adopted. The efficacy of a Periplaneta americana extract was validated by evaluating hair regrowth status and skin pathological staining in C57BL/6J mice. Transcriptomics, metabolomics, RT-qPCR, and 16s rRNA techniques were integrated to dissect the underlying mechanisms of its hair-growth-promoting effects. PA-011 significantly promoted hair regeneration in depilated mice via multiple mechanisms: enhanced skin superoxide dismutase activity and upregulated vascular endothelial growth factor expression; modulated FOXO/PI3K/AKT signaling pathway and restored skin microbiota homeostasis; and accelerated transition of hair follicles from the telogen to anagen phase. PA-011 exerts hair-promoting effects through synergistic modulation of FOXO/PI3K/AKT signaling and the skin microbiome. As a novel therapeutic candidate, it warrants further systematic investigation for clinical translation.
脱发是一种影响全球半数以上人口的常见皮肤病，与心理困扰密切相关。药用昆虫资源美洲大蠊（Periplaneta americana, L. PA）的提取物具有药理活性（如抗氧化、抗炎、改善微循环），与脱发的核心治疗靶点一致。本研究旨在系统研究美洲大蠊提取物促进毛发生长的疗效和机制。采用网络药理学预测与体内实验相结合的策略。通过评估C57BL/6J小鼠的毛发生长状态和皮肤病理染色，验证了美洲大蠊提取物的疗效。整合转录组学、代谢组学、RT-qPCR和16s rRNA技术，剖析其促进毛发生长作用的潜在机制。PA-011通过多种机制显著促进脱毛小鼠的毛发生长：增强皮肤超氧化物歧化酶活性并上调血管内皮生长因子表达；调节FOXO/PI3K/AKT信号通路并恢复皮肤微生物群稳态；加速毛囊从休止期向生长期的转变。PA-011通过对FOXO/PI3K/AKT信号和皮肤微生物群的协同调节发挥促进毛发生长的作用。作为一种新型治疗候选物，它值得进一步进行系统研究以实现临床转化。
