Cakmak Gamze, Soyguder Zafer
Department of Anatomy, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey.
Vet Med Sci. 2025 Mar;11(2):e70289. doi: 10.1002/vms3.70289.
In this study, white and grey matter volume densities of the lumbosacral part of spinal cord in ducks (Anatinae) were investigated by a stereological method. Ten healthy ducks weighing 3-4 kg, regardless of gender, were used as material. Anesthetized animals were perfused with 10% buffered formaldehyde. The lumbosacral parts of the ducks were exposed by dissecting the spinal cords. The lumbosacral section was segmented. Tissue samples from each segment were determined. A total of 12 sections were taken from these tissue samples, each 250th section with a thickness of 5 µm on the microtome. These sections were stained with haematoxylin-eosin. Photos were taken with an X10 objective. By using Cavalieri's Principle, volume density calculations of both the whole tissue and the white and grey matter were performed separately in each segment of the lumbosacral part of the spinal cord in ducks. Shtereom I program was used for calculations. As a result, the white matter and grey matter volume values of all tissue in the duck lumbosacral segments and their volume fractions with each other were determined and evaluated in the light of the literatures. In this study, when all the volume values of the lumbosacral spinal segment in duck were revealed, it was determined that the volume value increased in the range of LS3 and LS8 segments. The increase in the whole volume value in these segment ranges in ducks gave rise to the idea that the enlargement called intumescentia lumbosacralis may be between these segments.
在本研究中，采用体视学方法对鸭（鸭科）脊髓腰骶部的白质和灰质体积密度进行了研究。选用10只体重3 - 4千克、不分性别的健康鸭作为材料。对麻醉后的动物灌注10%的缓冲甲醛。通过解剖脊髓暴露鸭的腰骶部。对腰骶部切片进行分段。测定每个段的组织样本。从这些组织样本中总共取12个切片，在切片机上每隔250个切片取一个，厚度为5微米。这些切片用苏木精 - 伊红染色。用10倍物镜拍照。利用卡瓦列里原理，分别对鸭脊髓腰骶部各段的整个组织以及白质和灰质进行体积密度计算。计算使用Shtereom I程序。结果，确定了鸭腰骶段所有组织的白质和灰质体积值及其相互之间的体积分数，并根据文献进行了评估。在本研究中，当揭示出鸭腰骶脊髓段的所有体积值时，确定在LS3和LS8段范围内体积值增加。鸭在这些段范围内总体积值的增加引发了这样一种观点，即所谓的腰骶膨大可能在这些段之间。
