Johnson P
Res Vet Sci. 1985 May;38(3):329-33.
An unusual compound of small molecular weight has been detected in serum from horses with acute grass sickness by solvent extractions and thin-layer chromatography. The substance has not been detected in the serum of normal horses or cases of equine colic and apparently disappears from grass sickness serum after two to three days of clinical illness. Although this compound is found in sera which are known to possess in vivo neurotoxicity, this property could not be demonstrated in either the total chloroform extract which contains the compound or in the hydrophilic serum components remaining after extraction. These findings suggest that, although the compound may be related to the neurotoxin present in the original serum, the toxicity is inactivated during the extraction procedure or that the emulsion necessary for administration precludes absorption of an active toxin.
通过溶剂萃取和薄层色谱法，在患有急性青草搐搦的马匹血清中检测到一种分子量较小的异常化合物。在正常马匹或马属绞痛病例的血清中未检测到该物质，并且在临床发病两到三天后，该物质明显从青草搐搦血清中消失。尽管在已知具有体内神经毒性的血清中发现了这种化合物，但在含有该化合物的总氯仿提取物或提取后剩余的亲水性血清成分中均未证实这种特性。这些发现表明，尽管该化合物可能与原始血清中存在的神经毒素有关，但毒性在提取过程中失活，或者给药所需的乳剂阻止了活性毒素的吸收。
